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Construction and Prevalidation of V79 Pathway Cell Lines: Heterologous Co-Expression of human Cytochrom P450 1A2 and polymorphic forms of human N-Acetyltransferase in V79 Chinese Hamster Cells for in vitro-studies on toxicity and metabolism of chemicals and drugs.
Project
Project code: BfR-ZEBET-08-1328-166
Contract period: 01.10.2001
- 30.09.2004
Purpose of research: Applied research
V79 cells were genetically engineered for stable co-expression of human cytochrome P450 and genetic variants of human N-Acetyltransferase. Both enzymes together handle aromatic amines, e.g. 2-aminofluorene and other structurally related chemicals. As for all chemicals and drugs it is of fundamental importance to understand metabolism in order to identify metabolites which are responsible for toxicological and pharmacological consequences. The newly constructed cell lines are a perfect tool to investigate and understand metabolic activation of aromatic amines. The genetechnological approach is perfect for substituting animal experimentation and at the same time may generate data of immediate relevance for humans and in that way, allow for a more realistic risk assessment.
Section overview
Subjects
- Biotechnology
Framework programme
Funding programme
Excutive institution
BfR - Centre for Documentation and Evaluation of Alternatives to Animal Experiments (BfR - ZEBET)