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Decreasing the dissemination of antibiotic resistance genes by targeted hygiene measures (Decreasing the dissemination of antibiotic resistance genes)
Project
Project code: kA
Contract period: 20.05.2015
- 30.06.2017
Budget: 81,368 Euro
Purpose of research: Applied research
Keywords: antibiotic resistances
Antibiotic resistance gens can be transferred horizontically in bacterial communities even between different species. The intensifying effect of an elevated density of microorganisms f.e. in biofilms, on the horizontal gene transfer rate has been demonstrated for several habitats. In this project, we want to demonstrate the correlation between density of the microbial community and the prevalence of antibiotic resistance genes. In previous studies we could demonstrate that for milking machines distinct parts of the machine show an increased load of microorganisms. For those parts, the portion of antibiotic resistant bacteria related to the total microbial community will be analyzed and compared with less contaminated parts of the machine. In parallel with the cultivation-dependent approach a direct quantification of resistance genes and reference genes will be performed by quantitative real-time PCR. A correlation analysis of these quantitative data will reveal the relationship between density of microorganisms and presence of resistance genes. The mobilization of resistance genes in high density microbial populations will be confirmed by in-vitro experiments using authentic isolates from the analyzed milking plants.
Section overview
Subjects
- Food microbiology