We use cookies on our website. Some are necessary for the operation of the website. You can also allow cookies for statistical purposes. You can adjust the data protection settings or agree to all cookies directly.
Data Protection Declaration
Imprint
Genetic analysis of wheat resistance to furoviruses Soil-borne cereal mosaic virus (SBCMV) and Soil-borne wheat mosaic virus (SBWMV)
Project
Project code: JKI-EP-08-2241
Contract period: 01.10.2013
- 30.09.2015
Purpose of research: Applied research
On the basis of new reports about the occurrence of soil-borne viruses in wheat expects that furoviruses are continue occurred as known up to now. For this reason the wheat breeding is confronted with new tasks to identify new resistance sources in wheat for integration in the development of virus resistant cultivars because soil-borne viruses cause high yield losses.
In breeding programs varieties and breeding lines with resistance to Furoviruses were identified, but information about the mechanism and genetics of resistance is limited, and sometimes even contradictory. For the SBCMV/SBWMV resistance in hexaploid wheat the Sbm1 locus was mapped to chromosom 5DL and the Sbm2 locus to chromosom 2BS. Diagnostic markers were developed. For other resistances (for example, further QTL) no molecular markers are available to date. Therefore, the aim of the project undertaken by the Institute of Resistance Research and Stress Tolerance (RS) and the Institute of Epidemiology and Pathogen Diagnostics (EP) was to develop diagnostic markers for resistance genes. The part of the project executed by EP consisted in the phenotypic analysis of the resistance to Furoviruses in the progeny of crosses of populations with the Sbm1 locus, the Sbm2 locus and an unknown resistance. The evaluation of resistance reactions was carried out after virus transmission to wheat seedlings by the vector Polymyxa graminis in infectious soil under glasshouse conditions. Experiments were carried out for the following populations: 1. Emerald (s) x Primus (r) (Sbm2 locus) The results of the phenotypic analysis showed that the Sbm2 gene was not effective against SBWMV. Of the 187 DH lines tested 183 (98%) were infected with SBWMV. 2. PI566671 (r) x Mirage (r), - x Tremie (r), - x Centrum (s) Despite the presence of a resistant phenotype in PI566671, none of the known resistance markers (eg Sbm1) were detected in this wheat. The reason for this could be that this wheat source is based on a cross with the intermediate wheatgrass Thinopyrum intermedium, which is not identified by the marker analysis. 3. Tremie (r) x Texel (s) -HOV1 population (Sbm1 locus) In the progeny of this cross (DH lines HOV1) the Sbm1 gene seems to have only a limited effect against SBWMV. The susceptible standards Saissons and JB Asano are 100% infected by SBWMV.The susceptible parent Texel was 86% infected, the resistant parent Tremie - 64%. Regarding SBWMV resistance we are dealing here with a cross of partial resistance (pr) x susceptibility (s). 4. Claire (r) x Savannah (s) population (Sbm1 locus) The susceptible standard JB Asano and the susceptible parent Savannah were 100% infected by SBWMV. The resistant parent Claire was 59% infected. This means there is again a cross between partial resistance (pr) x susceptibility (s) for SBWMV. From the results of the resistance tests in the progeny of different crosses it is concluded that the known Sbm1 and Sbm2 resistance genes do not control the resistance against SBWMV in wheat. The isolates of SBCMV from Germany are very aggressive for rye and triticale, and little infectious for wheat. The impact of the Sbm1 gene in wheat against SBCMV needs to be evaluated with a virus population highly aggressive for wheat, for example in Vatan, France. In the event that both genes are effective in conveying resistance in wheat against SBCMV, differences in resistance between the two Furoviruses need to be examined.
Section overview
Subjects
- Plant Breeding
- Crop Protection
Framework programme
Funding programme
Excutive institution
Institute for Epidemiology and Pathogen Diagnostics (JKI-EP)
