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Data Protection Declaration
Imprint
BfR - Centre for Documentation and Evaluation of Alternatives to Animal Experiments (BfR - ZEBET)
Institution
Section overview
Description
ZEBET is the 'Centre for Documentation and Evaluation of Alternative and Complementary Methods to Animal Experiments 'at the BfR. It was founded in 1989 with the aim to restrict the use of animals for scientific purposes to the absolute minimum and to develop alternatives to animal testing. ZEBET since 1994 is composed by a committee to advise representatives of science, industry, animal welfare organizations and state agencies.
The goal of the scientific animal welfare will be achieved through the 3Rs principle:
1) Replace - avoidance of animal testing through the use of alternative methods
2) Reduce - reduce the number of animals to the minimum
3) Refine - reducing the suffering of animals
ZEBET researched, developed and validated in our laboratory, alternative methods based on the 3R principle, mainly in the range of statutory, toxicological testing. She has achieved particular success in the development of alternative methods of field
1) the phototoxicity
2) the toxicity of skin and eye
3) the developmental toxicity using embryonic stem cells.
This method examines the potential of a substance, growth and development of organisms affecting.
Activities
- Research
Parent institution
Coordinated projects
- Actions of Botulinum Toxin A in nerve-muscle-tissue-cultures: A proof-of-principle-study
- Alternative methods - Joint research project: 3R-Schooling for Methodological approaches to Reduce Animal Tests. Subproject 3
- Best Search Practice on Animal Alternatives - A Guide for Untrained Database Users
- Biotechnological derived skin models for the cutaneous uptake of steroid hormone formulations
- Cell culture models of human nasal mucosa for in-vitro drug absorption studies (follow-up project to 1328-469)
- Characterisation and prevalidation of a new human cornea equivalent model for the evaluation of ocular toxicity
- Combination of a microphysiologic system and a scaffold-free 3D organoid as a developmental model for ossification
- Computerised risk assessment of dermal absorption of active agents
- Construction and Prevalidation of V79 Pathway Cell Lines: Heterologous Co-Expression of human Cytochrom P450 1A2 and polymorphic forms of human N-Acetyltransferase in V79 Chinese Hamster Cells for in vitro-studies on toxicity and metabolism of chemicals and drugs.
- Database search for the development of a multiorgan chip as an alternative to animal experiments with repeated dose toxicity testing
- Development and evaluation of alternative methods to replace animal experiments (NIH-Test) for the potency measure of vaccines against rabies
- Development and Validation of an Inductive Database for the Prediction of Carcinogenic Effects of Chemicals
- Development of a biotransformation system for the metabolic activation of validated in vitro systems to study embryotoxicity
- Development of a novel approach in hazard and risk assessment of reproductive toxicity by a combination and application of in vitro, tissue and sensor technologies - ReProTect
- Development of a single cell model of adult cardiomyocytes as a replacement method of animal experiments? Optical measurements of excitation-contraction coupling under the influence of test substances
- Development of an in vitro model (skin culture from slaughtered animals) to study the influence of compounds on wound healing.
- Development of bioreactors for in vitro testing of biological heart valves for reduction of animal experiments
- Development of methods for detection of obesogenic substances
- Development of methods for the isolation and analysis of Yessotoxin
- Development of Predictive In Vitro Test for Developmental Neurotoxicity testing
- Embryonic stem cell-based novel alternative testing strategies (ESNATS)
- Equine precision cut lung slices as an in vitro model of obstructive airway diseases for the identification and characterization of drug targets
- Establishment of a stem cell-based system for the systematic testing of negative side-effects of drugs on human cardiomyocytes
- Establishment of normal human neural progenitor cells as an in vitro screening model for the evaluation of the neurotoxic potential of chemicals
- Estimating the risk of substances by spatial multi-biomarker profiling of 3D tissue cultures
- Evaluation of Alternative Methods to Animal Experiments in Life Sciences
- Evaluation of optimal transport condition for the shipping of human organotypic corneal equivalents for in vitro drug absorption studies
- Further development of a 3D-model of substance distribution in the cochlea after round window application
- Gastric cell lines to substitute native cells of the gastric gland to study acid secretion in special consideration of preclinical studies
- Generation of three dimensional vascular tissues and establishment of long-term cultures for establishing a multi-organ chip as an alternative to repeated dose (systemic) toxicity testing in vivo
- High-throughput methods for the estimation of toxicological effects of nanomaterials
- HPCT-1E3 hepatocytoma cultures for determination of cytotoxicity, toxicokinetic interactions and cholestatic effects, pre-validation study
- Human organotypic corneal equivalents for in-vitro drug absorption and metabolism studies
- Identification of electrophysiological endpoints in stem cell-based models for developmental neurotoxicity assays
- Improvement of an in vitro stem cell assay for developmental toxicity: Establishing molecular endpoints of tissue specific development
- Improvement of the embryonic stem cell test (EST) using neural marker genes for testing the developmental neurotoxicity in vitro
- Improving the gain of transgenic mouse embryos for cryopreservation
- In vitro exposure of lung cells with aerosol at the air-liquid-layer as an alternative for inhalation experiments with animals
- Joint Project: Development of an in vitro embryo toxicity tests with mouse embryonic stem cells: Subproject 1 - Analysis of embryotoxic effects in the light of new endpoints and metabolism using an expanded selection of materials
- Joint project: Pluripotent stem cells in automated prediction of developmental osteotoxizität. Subproject 3: Development and establishment of a Osteoblsten differentiation assays
- List of chemicals with missing IC 50-values and/or LD 50-values
- Mechanisms of Critical Illness-induced Cognitive Dysfunction
- Normal human neural progenitorcells as a screening model for substances that are toxic for neural development
- Optical probe technique for the assessment of multidrug resistance profiles in 3-dimensional in vitro tumor models
- Optimizing the pain management in the mouse-osteotomy-model – Integration of a refinement study in a basic research study
- Organotypic cell culture models of human nasal mucosa for in-vitro drug absorption studies
- Porcine Lungs from the Slaughtering Process as a Model for Ex Vivo Lung Perfusion (EVLP)
- Predictive cardiotoxicity testing based on human stem cell-derived cardiomyocytes
- Preparation for external prevalidation: Optimization of the internally validated and standardized method for quantitation of angiogenesis and antiangiogenesis in vitro by determination of the angiogenic standard of the cells to be used
- Prevalidation of human cornea model based on immortalized corneal cell lines as in vitro alternative to the Draize eye test.
- Refinement methods to reduce laboratory animal suffering: A review of German biomedical and animal research applications from 2010 to identify how refinements are used in experiments involving rodents
- Refinement of the LCSA (loose-fit coculture-based sensitization assay)
- Replacement of fetal calf serum in the embryonic stem cell test (EST) – Standardization of culture conditions for proliferation and differentiation of embryonic stem cells of the mouse
- Severity assessment from an animal's point of view
- siRNA technology in mammalian cells as alternative of knock-out mice: Functional investigations of proteins of the Myc-Miz-1-TopBP1-network (translation).
- Studies on the anti-oxidative responses of lung cells as an endpoint for the assessment of aerosols after exposure at the air-liquid interface
- The isolated perfused bovine udder as an in vitro model for the evaluation of sensibilisation/sensitisation induced by chemicals
- The role of endocrine active substances and hormone receptors for centrosome amplification-induced chromosomal instability in colorectal cancer
- Use of transgenic animals in biomedical research in Germany - survey 2001 to 2003 and perspectives to change research to non-animal test methods
- Using phosphoprotein profiling for the characterisation of effects of teratogenic substances on essential signal transduction pathways during the differentiation of mouse embryonic stem cells
Involved in research projects
- Characterization of metabolizing enzymes and biotransformation of selected test substances in human in-vitro skin models to identify an optimal test system for dermato-toxicological and exposure-related studies
- Prevalidation for testing the toxic effects of inhaled active substances (gases) after direct exposure of lung cells of people in the Luft-/Flüssigkeitsgrenzschicht
Contact
BfR - Centre for Documentation and Evaluation of Alternatives to Animal Experiments
(BfR - ZEBET)
Diedersdorfer Weg 1
12277 Berlin
Berlin
Germany
Phone: +49-(0)30-8412-4300
Fax: +49-(0)30-8412-4970
Email: zebet(@)bfr.bund.de
