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Enhanced control of CBPP in sub Saharan Africa through development of better diagnostics and vaccines

Project

Food and consumer protection

This project contributes to the research aim 'Food and consumer protection'. Which funding institutions are active for this aim? What are the sub-aims? Take a look:
Food and consumer protection


Project code: FLI-IMP-08-Je-0044
Contract period: 01.05.2010 - 30.10.2013
Purpose of research: Basic research
Keywords: epizootic diagnostic tests, contagious bovine pleuropneumonia transmission models

The goal of the project is to develop and make available better diagnostic tests and a new vaccine for contagious bovine pleuropneumonia (CBPP), which are significantly better than those currently available. The data obtained as part of the validation of the improved tests will also enable existing epidemiological models of CBPP transmission to be enhanced. The improved epidemiological models will enable the identification of best-bet options for use of the new and more effective diagnostic test, leading to effective control (perhaps eventual elimination) of the disease in Africa, and to the formulation of new, more appropriate and evidence-based control policies. The current project will generate a number of new outputs: some will have immediate application, while others are significant intermediate products which will represent major advances towards the development of a new improved vaccine and perhaps also a point-of-care (pen-side) diagnostic test. All intermediate products will be placed in the public domain and their use and further development will be actively encouraged and facilitated to maximize the probability of the long-term goal being achieved. The main output with immediate application will be a laboratory diagnostic test that will provide greater sensitivity and specificity than the currently available test. This will be achieved by pursuing a systematic search and validation of antigenic specificities that appear in different phases of the infection to develop assays that can detect infected cattle in all stages of diseases, including chronic carriers. The main intermediate output will be the identification of pathogen molecules that could be used for designing more effective vaccines. That will be achieved by comparing the in vitro and in vivo proteome of Mycoplasma mycoides subsp. mycoides SC (MmmSC) and by functional inhibition studies using a panel of well defined monoclonal antibodies (MAbs). The main task of the FLI in Jena is to develop a sensitive prototype diagnostic test. Most of this work will be carried out by the German National Reference Laboratory for CBPP at the FLI in Jena. The antigens delivered by ILRI will be tested for their presence in other MmmSC strains and related mycoplasma species using the strain collection of the national reference laboratory. FLI will determine the occurrence and conservation of the encoding genes using PCR, hybridization and sequencing techniques. FLI in Jena will also establish an ELISA to assess the sensitivity and specificity of individual antigens using sera from 100 experimentally infected cattle and to determine the dynamics of the individual antibody responses over time. An important objective is to deliver a ‘gold standard’ laboratory assay that is capable of detecting all infected animals. Activities: Activity 1: Reassess necessary specifications for an improved diagnostic test (ILRI) Activity 2: Screening of potential diagnostic antigens (ILRI) Activity 3: Development of a sensitive prototype diagnostic test (FLI Jena) Activity 4: Assessment of CBPP burden in selected areas in Namibia and Kenya (Kenya Agricultural Research Institute, Central Veterinary Laboratory Windhoek) Activity 5: Exploring the potential of point-of-care tests (ILRI) Activity 6: Establishment of a panel of monoclonal and polyclonal antibodies (ILRI) Activity 7: Inhibition tests using MAbs (ILRI) Activity 8: Comparative analysis in vivo and in vitro proteome of mycoplasma documented (ILRI, Tierärztliche Hochschule Hannover) Activity 9: Evaluating the diagnostic tools in CBPP transmission models (ILRI).

Heller M, Gicheru N, Tjipura-Zaire G, Muriuki C, Yu M, Botelho A, Naessens J, Jores J, Liljander A. 2016. Development of a novel cocktail enzyme-linked immunosorbent assay and a field-applicable lateral-flow rapid test for diagnosis of contagious bovine pleuropneumonia. J Clin Microbiol 54:1557–1565. doi:10.1128/JCM.03259-15.

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Framework programme

BMEL Frameworkprogramme 2008

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