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Evaluation of Malus genetic resources on tolerance to ARD
Project
Project code: JKI-ZGO-08-4189, 4189
Contract period: 01.01.2016
- 31.12.2018
Purpose of research: Applied research
Keywords: ARD-tolerant rootstocks, genetic ressources
The commonly dwarfing apple rootstocks used in high-density orchards are susceptible to ARD. The utilization of resistant or tolerant rootstocks would be a sustainable and economic solution for growers. Research on ARD-resistant or tolerant rootstocks has received little attention as well as the mechanisms underlying resistance. The project is focused on the evaluation of apple germplasm obtained from the JKI Genebank in order to utilize as potential source of effective resistance or tolerance to ARD in breeding. Seedling lots and clonal accessions of 20 Malus species selected previously based on resistance to fire blight as well as rootstock selections including such from the Geneva rootstock breeding program will be chosen for tolerance screening. The evaluation will be carried out in greenhouse based on containers prepared with soil from the JKI orchard in Pillnitz with known replant problems and cultivation of apple trees since 1920 (infected), and disinfected soil (control). Sources of genetic tolerance to ARD will be identified (Phase 1 and 2 of the project) and used to create segregating populations in order to localize the genomic background of resistance / tolerance (end of Phase 2 and Phase 3). Furthermore, a transcriptome analysis using a RNA sequencing (RNAseq) approach will be performed using replant soil and disinfected soil and comparing a susceptible apple rootstock, like M9, and a tolerant genotype. This experiment will be realized in cooperation with other partners of the ORDIAmur research project.
Section overview
Subjects
- Plant Breeding
- Crop Protection
- Arboriculture
- Genetic Resources
