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Improving stem rust resistance in rye by genetic and molecular tools

Project

Production processes

This project contributes to the research aim 'Production processes'. Which funding institutions are active for this aim? What are the sub-aims? Take a look:
Production processes


Project code: JKI-A-08-1207, 156 E
Contract period: 01.02.2017 - 31.01.2019
Purpose of research: Applied research

Stem rust (Puccinia graminis f. sp. secalis) is one of the most destructive diseases in rye (Secale cereale L.) causing yield losses of about 30 to 50 % in many regions/countries including Germany, Poland, Belarus and Russia. We aim for (1) virulence analysis of stem-rust populations in Germany and Poland to monitor race composition and complexity as a prerequisite to estimate durability of resistance, (2) establishment of a diversity panel of rye populations with a varying degree of stem rust resistance, (3) identification of new stem rust resistance genes by leaf-segment tests in the lab, (4) molecular analysis of segregating rye populations for adult-plant stem rust resistance in the field, and (5) association mapping with foreign germplasm resources. These innovation targets will result in (a) defined, highly virulent stem rust races necessary for resistance testing, (b) new, effective resistance genes that can be used in breeding programs and (c) linked molecular markers enabling the breeding companies to efficiently select for stem-rust resistances in the adult-plant stage.

The rye stem rust population in Germany has been tested for virulence frequency, complexity and diversity. At eight sites in Germany and Poland, field trials for the selection of stem rust resistance in the adult plant stage took place. In addition to the field trials, leaf segment tests were performed to identify new race specific genes in inbred lines and genetic resources. The assessment of resistance in seedling and adult plant stage is an essential basis for future selection in the breeding companies. The first result s were presented during the rust workshop in Berlin-Dahlem and the resistance conference in Fulda.

Analysis of German and Polish rye stem rust populations in 2017 and 2018 showed an extremely high diversity, but 3 of the 15 differential lines tested remained uninfected. At eight sites in Germany and Poland, field trials for selection of stem rust resistance in the adult plant stage took place. ln addition leaf segment tests were performed to identify new race specific genes in inbred lines and genetic resources. The genes responsible for these resistances, could be localized in the genome with the help of molecular markers. The assessment of resistance in seedling and adult plant stage is an essential basis for Future selection in the breeding companies.

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Subjects

Framework programme

BMEL Frameworkprogramme 2008

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