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Identification of Wheat dwarf virus tolerance in the barley genepool and their exploitation for breeding (VIRTOGE)

Project


Project code: 2818201315, JKI-RS-08-3403
Contract period: 01.11.2016 - 31.10.2019
Budget: 255,530 Euro
Purpose of research: Applied research

The project aims at the identification of Hordeum genotypes with tolerance to wheat dwarf virus (WDV) by screening of H. vulgare / H. bulbosum accessions using artificial virus inoculation in a gauzehouse and infection under natural conditions. Futhermore, the accessions are genotyped by the newly developed 50 k iSelect chip based on phenotypic and genotypic data QTL for WDV-tolerance will be identified by genomewide association mapping (GWAS). KASP-marker will be developed based on respective SNPs as a prerequisite for marker-assisted introgression of WDV-tolerance in adapted genotypes. Results: - Evaluation of a set of barley genotypes for WDV-tolerance with artificial WDV-infection in a gauzehouse and under natural conditions at 3 locations. Selection of tolerant accessions (1st to 3rd year). - Phenotypic characterisation of accessions differing in WDV-tolerance for agronomic traits, use of these additional traits for GWAS (2nd aund 3rd year). - Crossing of tolerant accession with high yielding cultivars/breeding lines, production of DH populations (begin 2nd year). - Genotyping of 250 tolerant and non-tolerant accessions by using the 50k iSelect Chip to detect polymorphic SNP-marker. GWAS to identify QTL for WDV-tolerance and further agronomic important traits. Development of KASP-markers (3rd year). - Verification of QTL in segregating DH-populations (4th and 5th year).

In the first year, the aim of the project is to identify promising WDV resistant/tolerant sources by screening of 250 barley accessions from different species and geographic regions, using artificial virus inoculation with Psammotettix alienus in a gauzehouse. First results of WDV detection by ELISA showed that in October 2016 virus inoculation was insufficient due to bad weather conditions (low temperatures/high precipitation). For this reason, scheduled resistance test of approximately 150 barley accessions is going to be repeated in the greenhouse.

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