Logo of the Information System for Agriculture and Food Research

Information System for Agriculture and Food Research

Information platform of the Federal and State Governments

Identification of Wheat dwarf virus tolerance in the barley genepool and their exploitation for breeding (VIRTOGE)


Project code: 2818201315, JKI-RS-08-3403
Contract period: 01.11.2016 - 31.10.2019
Budget: 255,530 Euro
Purpose of research: Applied research

The project aims at the identification of Hordeum genotypes with tolerance to wheat dwarf virus (WDV) by screening of H. vulgare / H. bulbosum accessions using artificial virus inoculation in a gauzehouse and infection under natural conditions. Futhermore, the accessions are genotyped by the newly developed 50 k iSelect chip based on phenotypic and genotypic data QTL for WDV-tolerance will be identified by genomewide association mapping (GWAS). KASP-marker will be developed based on respective SNPs as a prerequisite for marker-assisted introgression of WDV-tolerance in adapted genotypes. Results: - Evaluation of a set of barley genotypes for WDV-tolerance with artificial WDV-infection in a gauzehouse and under natural conditions at 3 locations. Selection of tolerant accessions (1st to 3rd year). - Phenotypic characterisation of accessions differing in WDV-tolerance for agronomic traits, use of these additional traits for GWAS (2nd aund 3rd year). - Crossing of tolerant accession with high yielding cultivars/breeding lines, production of DH populations (begin 2nd year). - Genotyping of 250 tolerant and non-tolerant accessions by using the 50k iSelect Chip to detect polymorphic SNP-marker. GWAS to identify QTL for WDV-tolerance and further agronomic important traits. Development of KASP-markers (3rd year). - Verification of QTL in segregating DH-populations (4th and 5th year).

In the first year, the aim of the project is to identify promising WDV resistant/tolerant sources by screening of 250 barley accessions from different species and geographic regions, using artificial virus inoculation with Psammotettix alienus in a gauzehouse. First results of WDV detection by ELISA showed that in October 2016 virus inoculation was insufficient due to bad weather conditions (low temperatures/high precipitation). For this reason, scheduled resistance test of approximately 150 barley accessions is going to be repeated in the greenhouse.

show more show less


Advanced Search