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Imprint
Toxic impact of contaminants assessed with the zebrafish embryo model system
Project
Project code: TI-FI-08-PID1607
Contract period: 01.01.2001
- 31.12.2020
Purpose of research: Experimental development
The zebrafish (Danio rerio) is one of the favourite organisms for developmental biologists and for toxicologists.The embryonal stage of this fish is completed in a few days and can be observed easyly due to the transparent chorion. The early life stage test with zebrafish eggs can help to assess toxic impact of pollutants by orbservation of developmental abberations in the embryo. Briefly, fertilized and well-developed eggs in the 4 to 8 cell stage were selected for the test and placed in 24-well dishes. In each well 5 eggs were exposed in 1 mL test solution containing DMSO and water. 60 eggs were used for each sample (known concentration of contaminant, positive or negative control). Incubation took place at 26°C for 48 h. After that Eggs were monitored for coagulation and malformations using an inverse microscope. Lethal (coagulation, missing heartbeat, missing somites, missing tail detachment from the yolk sac, missing spontaneous movement) and non-lethal malformations (yolk sac edema, spine deformation, no pigmentation, incomplete eye development) were reported separately. The test ist repeated twice
Section overview
Subjects
- Animal health
- Marine fisheries
- Biotechnology
