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Genomcs-driven analysis of the wheat/Fusarium pathosystem for healthy food and feed product development (FusResist)

Project


Project code: JKI-RS-08-3389
Contract period: 01.07.2015 - 30.06.2018
Purpose of research: Basic research

The aim of the project is the application of genome based and proteomic methods for (1) the selection for resistance against Fusarium (FHB) and for reduced mycotoxin production, (2) the analyses of the host-pathogen-interaction wheat/fusarium, (3) population and association genetics studies on Fusarium graminearum isolates.

Wheat is the cereal with the largest acreage world wide and of prime importance for human nutrition. Fusarium Head Blight (FHB), caused by the fungal pathogens Fusarium graminearum and Fusarium culmorum, causes yield losses, but especially a reduction in quality due to the production of mycotoxins, like Deoxynivalenol (DON) limiting the use for feed and food purposes. The aim of this collaborative project is the selection for resistance against FHB and for reduced mycotoxin production, the analyses of host-pathogen-interaction, and population and association genetics studies on Fusarium graminearum isolates. Studies conducted at the JKI aim at the construction of a high resolution mapping population for a FHB-QTL located on chromosome 2A of Triticum monococcum followed by marker saturation and the identification of candidate genes. The project contributes to broadening the genetic base of resistance to FHB by using genetic resources thereby conditributing to a consumer friendly and environmental sound wheat production. In 2016 the high-resolution mapping population could be increased to 1991 F2-plants, providing a genetic resolution of 0.025 % recombination. 421 heterozygot recombinant plants and 16 homozygous recombinant plants could be identified for the first QTL-Intervall and 242 heterozygous recombinant plants and 7 homozygous recombinant plants could be selected for the second QTL-Interval. Out of these recombinant plants 110 segmental recombinant inbred lines were developed. 9 markers were selected from the genetic map of Triticum monococcum so far for marker saturation and the development of a first high-resolution map. Additional markers are selected from Genotyping-by-sequencing or the reference map of Triticum aestivum in 2017.

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