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GABI-EVAST: EValuation of the power of ASsociation Tests versus QTL mapping
Project
Project code: 0313122B
Contract period: 01.07.2004
- 30.06.2007
Budget: 311,514 Euro
Purpose of research: Basic research
In this project the viability of association testing for the identification of candidate genes / genomic segments will be addressed and rigorously tested in the model system Arabidopsis. The application of cutting edge genotyping tools will be combined with the use of a multilevel phenotyping platform, which allows parallel measurements of metabolic traits and the activities of a large number of enzymes. These tools, which were developed in GABI-1, will be applied to approach two important traits that are of fundamental importance for plant productivity – (i) starch accumulation and (ii) the balance between carbon and nitrogen-containing components (here termed ‘C/N interactions’). Using Arabidopsis natural accession-derived lines, F2 and RIL-populations, (i) association tests and (ii) QTL mapping will be performed using candidate cardinal genes and molecular markers. Furthermore, the viability and prospects of a novel procedure for association studies (involving gene / genome analysis of F2 phenotypic extremes) will be analysed. This will be done because it is suspected that, due to the structure of populations in autogamous plant species or in strongly selected breeding lines, gene mixing may be less complete than in e.g., human populations, and that considerable variation may be masked by counterbalancing epistatic interactions between genes and will only become visible after outcrossing parental lines.
Section overview
Subjects
- Plant Breeding
Framework programme
BMBF - Frameworkprogramm Biotechnology - Using and Shaping its Opportunities
