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Functional characterization of terpensythases from carrot
Project
Project code: JKI-SG-08-2230
Contract period: 01.10.2018
- 31.10.2020
Purpose of research: Experimental development
Keywords: carrot, terpene synthases, breeding, metabolite analysis
This project is aimed at functional characterization of terpene synthases (TPS) from carrot. To this end, candidate TPS genes will be cloned and expressed in Escherichia coli and Nicotiana benthaminiana (tobacco). Intact proteins will be purified from E. coli and tested for their enzyme activity in vitro. Furthermore, the influence of these genes on the conversion and formation of individual terpenes or metabolically related derivatives will be investigated in vivo by Agrobacterium-mediated transient expression. Combination of these two approaches allow understanding of the functional diversity of individual TPS genes.
Section overview
Subjects
- Plant Breeding
- Crop Protection
- Vegetable Gardening
