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Legionella in agricultural exhaust air treatment plants. Subproject TI (LiLA)
Project
Project code: 28N200201
Contract period: 01.08.2020
- 31.07.2023
Budget: 304,316 Euro
Purpose of research: Applied research
Keywords: animal husbandry, environment and nature protection, hygiene, health-related consumer protection, stable building, pigs, husbandry techniques
In this project, suitability-tested and properly operated agricultural exhaust air treatment plants are to be examined for the presence of Legionella in order to be able to estimate the health risks to the population through the operation of these systems and to assess the need for action by lawmakers and law enforcement agencies to mitigate a potential risk. The following goals are pursued: • Recording the current status of exhaust air purification systems in livestock farming, which can potentially be contaminated with Legionella. • Risk assessment of exhaust air purification systems in livestock farming through regular sampling and examination for Legionella and amoeba in the process water and in the bio-aerosol-containing exhaust air (bio-aerosol) • Establishment of a quick and easy hygiene control using an automated microarray-based molecular biological rapid test (with integrated live / dead differentiation and upstream concentration method) for the culture-independent quantification of Legionella on site. • Evaluation of the measurement method with process water and bioaerosol samples from a wide variety of plants • Evaluation of the automated sandwich microarray immunoassay (LegioTyper) for rapid serotyping of Legionella pneumophila in agricultural exhaust air purification systems For this purpose, both a molecular biological isothermal rapid test for Legionella and an antibody-based rapid test for the identification of serogroups of Legionella pneumophila are established and analytically validated on the MCR R analysis platform. With this test the process water, biofilms and exhaust air from three representative properly operated ARAs are investigated for one year for Le-gionella. The planned method allows both the molecular biological quantification of bacteria of the genus Legionella and the health-relevant pathogen Legionella pneumophila, as well as molecular biological tests as to whether the bacteria found are viable or active or not, as well as antibody-based tests for the determination of serotypes of Legionella pneumophila that can differ in their pathogenicity. In a further step, this test will be used to investigate a representative cross-section of at least 25 ARA in Germany for Legionella. On the basis of this data, a risk assessment is created and a possible need for action is given.
Section overview
Subjects
- Animal Husbandry
- Agricultural Technology Animal Production
- Animal health
