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Meat juice multi serology - Development of a 'pig-specific microarrays' as a monitoring tool or for setting up a monitoring system with multi serological meat juice as sample material to minimize pathogenic / zoonotic pathogens in the food chain
Project
Project code: 2811HS013
Contract period: 01.03.2012
- 28.02.2013
Budget: 65,686 Euro
Purpose of research: Applied research
The objective of this study is to increase the informative value of the current food chain information by adding the meat juice multi-serology concept for providing bet-ter knowledge on the health status of pig herds for the food operators, veterinary authorities and pig producers. The consecutive testing of samples from pig herds using single ELISA tests is far too expensive for being considered as routine method used in monitoring systems. Therefore, miniaturized and simultaneous methods (one drop of specimen, several serological results at the same time), which produce cost-efficient meaningful results, i. e. targeted and continuously reproduced serological herd profiles for pig herds delivering pigs for slaughter, are needed. The methodological approach is: Various preparations and concentrations of the antigens of the pathogens relevant for food safety and for pig health such as Salmonella spp., Yersinia enterocolitica, Trichinella, Toxoplasma gondii, Mycoplasma hyopneumoniae, Actinobacillus pleuropneumoniae, and Influenza-A H1N1 and H3N2 will be spotted on blank microchips. After determining the optimum antigen structure and concentration for each antigen using meat juice samples from reference laboratories, prototype microchips will be produced. The resulting microarray prototype will be tested with field meat juice samples, for which the antibody concentrations are known due to their being previously tested with commercial single antigen ELISA tests.
Section overview
Subjects
- Animal health
- Biotechnology
- Food microbiology