Development of a molecular diagnostic for sustainable and resource-efficient production of the renewable resource potato starch. Subproject 1 (DiRK)

Project

Project code: 22036111
Contract period: 01.07.2013 - 30.06.2016
Budget: 188,679 Euro
Purpose of research: Applied research

The project aims at establishing precision breeding for starch potato varieties by means of DNA-based molecular markers. The main objective is the development and application of diagnostic markers for resistances to different pathotypes of the quarantine pathogen Synchytrium endobioticum (potato wart). Wart resistance will be combined with resistances to further quarantine pests like the potato cyst nematode Globodera pallida as well as immunity to potato virus Y. The intention is the future development of high yielding starch potato varieties to make sure the sustainable and profitable growth of starch potatoes as a local renewable plant resource for the starch processing industries. The following results have been achieved in the reporting period: WP1 Development of molecular markers for resistance to pathotypes 1,2,6,18: Extensive resistance tests were performed for the following groups of material: a) Potato varieties with known resistance spectrum: The inoculum for the tests of 47 varieties with multiple resistance, 50 varieties with P1 resistance, 50 varieties with P1 susceptibility and 80 wild species with unknown resistance or susceptibility to potato wart disease was increased for all four pathotypes (in total 15,900 warts). b) Segregating populations: 24 genotypes were infected with pathotype 1 and 18 genotypes with P18, seedling tissue was taken after 2, 7, 14 and 21 days and frozen, then shipped to partner LUH. c) Crossing populations with other donors of resistance: The parents of each crossing populations were tested with all four pathotypes (588 tubers). 150 genotypes of the population Panda x L04 were tested with 20 tubers on pathotype 18 and the distribution of the means was calculated. WP 3+4: Identification of candidate genes by proteom analysis of the infection course and mechanism of infestation course: JKI (AG Flath) has provided the partner LUH with P18 infected tuber material at regular intervals.

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