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Collaborative project: Identification of donors for effective restoration of male sterility based on cytoplasm of T. timopheevii and molecular characterization of P class PPR gene family as source of candidate genes for restoration - subproject 3

Project


Project code: 2814600613
Contract period: 01.10.2014 - 30.09.2017
Budget: 18,135 Euro
Purpose of research: Experimental development

Production of hybrid seeds using a cytoplasmic male sterility (CMS) system is successfully established in many crop species. In wheat, a male sterility system based on the cytoplasm of T. timopheevii is available, but effective restorer genes have yet to be identified. At the end of the project, a maximum number of two genes allowing the complete restoration of F1 seeds should be ready for use. Closely linked and diagnostic molecular markers will facilitate the efficient stacking of the restorer genes during breeding cycles. A candidate gene approach based on the P class PPR gene family will accelerate the screening of lines for restorer genes and enable the identification of effective restorers. The LfL and cooperating breeding companies will cross common wheat genotypes and genetic stocks carrying chromatin from wild wheat relatives with male sterile lines for surveying the presence of restorer genes. The teamwork of research institutes and breeding companies offers the exploitation of both a huge number and a wide range of germplasm. The breeding companies will concentrate in their work on adapted material from various countries and international agricultural research institutes, whereas the LfL will use its collection of various genetic stocks – synthetic, substitution and translocation lines. Segregating populations developed with successful restorer lines will be used for the genetic analysis of restorer genes. Based on known PPR gene sequences the gene family will be analysed in wheat by IPK. Allele mining should provide candidate genes for restoration and lines for initial field testing.

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Subjects

Excutive institution

KWS Lochow GmbH

Associated projects: Identification of donors for effective restoration of male sterility based on cytoplasm of T. timopheevii and molecular characterization of P class PPR gene family as source of candidate genes for restoration

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