New research projects in FISA http://www.fisaonline.de/ Here you will find the latest 20 projects that have been included in the Information System for Agriculture and Food Research (FISA). en-en Federal Office for Agriculture and Food TYPO3 Technical support to improve and automatize data collection and reporting on animal disease outbreaks and surveillance 1. Evaluation, review and advice on updating and harmonising the EFSA data model on AI, ASF and LSD and related population data 2. Survey of the data flow/structure within the Member States and identification of potential solutions to facilitate data preparation for EFSA or supranational databases 3. Exemplary implementation of such a data flow to EFSA for 4 Member States from different regions (e.g. CSF/ASF-WB-DB) 4. Overview of existing data analysis and reporting tools, including GIS, which could be used to facilitate the evaluation of the EFSA database 5. Implementation, if necessary adaptation and use of existing tools in the field of AI, ASF and LSD

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https://www.fisaonline.de/index.php?id=136&tx_fisaresearch_projects[p_id]=14321&tx_fisaresearch_projects[action]=projectDetails&tx_fisaresearch_projects[controller]=Projects&cHash=184e4577abcddb65cfeefe220d78dc77 Tue, 11 Aug 2020 06:02:13 +0000 https://www.fisaonline.de/index.php?id=136&tx_fisaresearch_projects[p_id]=14321&tx_fisaresearch_projects[action]=projectDetails&tx_fisaresearch_projects[controller]=Projects&cHash=184e4577abcddb65cfeefe220d78dc77
Stunning of large fish by percussion or captive bolt: development of operating procedures and evaluation of animal welfare aspects The aim of this project is to safeguard animal welfare during the slaughter of large fish specimens. To this end, the stunning of these fish by means of percussion or captive bolt will be evaluated with regard to effectiveness and animal welfare. Stunning of fish must ensure the induction of a long-lasting loss of perception, which allows the fish to be killed while still in a stage of insensibility. By means of the stunning procedures currently prescribed in Germany, this goal is difficult to achieve in some fish species. Some fish species have a low sensitivity to the stunning effect of electric current. During stunning of large fish specimens by percussion, however, a forceful blow has to be applied to the skull at a particular location. In BeFiBo, the force required for rendering fish insensible by percussion will be measured. Subsequently, a computer simulation model will be used to derive parameters that promise efficient stunning. In an identical manner, the application of a non-penetrating captive bolt device will be examined. For the stunning of sturgeon and catfish specimens over 25 Kg, stunning by means of a penetrating captive bolt device will be investigated as an alternative to stunning by percussion. In a laboratory study, indicators will be evaluated which will allow monitoring of the loss of consciousness of fish during the slaughter process on farms. The degree of distress or suffering related to the stunning process will be determined by measuring blood parameters. In co-operation with aquaculture farms, operating procedures will be developed for stunning fish by percussion or captive bolt, which will ensure effective stunning and safeguard animal welfare. The operating procedures will be made available to farm managers and official staff during training courses and as teaching material. In this way, legal requirements for stunning fish will be met and a high standard of animal welfare will be guaranteed in food fish production.

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https://www.fisaonline.de/index.php?id=136&tx_fisaresearch_projects[p_id]=14320&tx_fisaresearch_projects[action]=projectDetails&tx_fisaresearch_projects[controller]=Projects&cHash=aa54c0cbe23b7c1d55302b90d54f0d14 Mon, 10 Aug 2020 14:52:57 +0000 https://www.fisaonline.de/index.php?id=136&tx_fisaresearch_projects[p_id]=14320&tx_fisaresearch_projects[action]=projectDetails&tx_fisaresearch_projects[controller]=Projects&cHash=aa54c0cbe23b7c1d55302b90d54f0d14
Study on the improvement of pigs’ welfare during gas stunning in conventional paternoster and dip-lift systems by using alternative gases and gas mixtures The focus of this project to improve animal welfare at the slaughter of farm animals is the CO2 stunning of pigs. This stunning method causes less pre-slaughter stress on animals by driving them to the stunning facility in groups. However, when commonly used gas concentrations are applied, it leads to aversion, shortage of breath and escape reactions in the induction phase. This project will therefore investigate the use of alternative gases or gas mixtures for the stunning of slaughter pigs, which have a less aversive effect on the animal during the introduction phase and ensure that the animal remains unconscious and insensible until death by debleeding. In this field of research, several studies have already been carried out, some of which showed adverse effects of the application of alternative gases or gas mixtures on meat quality (especially hemorrhages when using argon and argon-nitrogen mixtures), but these effects are not fully understood. Broad studies on meat quality depending on the used gas are therefore intended to ensure a comparable product quality as possible. This meets the expectations of consumers for an animal-friendly treatment of livestock as well as for high-quality products. By using a newly developed gas technique, the gases and gas mixtures to be investigated can be applied in currently common gas stunning facilities using the dip-lift and paternoster systems, eliminating the need for an investment-intensive new construction of plants for the application of these gases. Due to the limited scope of the retrofitting of existing stunning facilities, a timely and economic implementation of the new gas stunning methods into practice after the end of the project seems to be feasible and an accelerated transfer of knowledge from research into practice can be achieved.

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https://www.fisaonline.de/index.php?id=136&tx_fisaresearch_projects[p_id]=14319&tx_fisaresearch_projects[action]=projectDetails&tx_fisaresearch_projects[controller]=Projects&cHash=97db4dfa62652928c1c8d5dee46d70b5 Mon, 10 Aug 2020 14:36:11 +0000 https://www.fisaonline.de/index.php?id=136&tx_fisaresearch_projects[p_id]=14319&tx_fisaresearch_projects[action]=projectDetails&tx_fisaresearch_projects[controller]=Projects&cHash=97db4dfa62652928c1c8d5dee46d70b5
Progress with competence and specialist knowledge for animal well-being, environmentally friendly and sustainable livestock farming TUM has the following goals in the project 1. Detection of the current status of exhaust air purification systems in livestock farming, which can potentially be contaminated with Legionella. 2. Establishment of a quick and easy hygiene control using an automated microarray-based molecular biological rapid test (with integrated live / dead differentiation and upstream concentration method) for the culture-independent quantification of Legionella on site. 3. Evaluation of the measurement method with process water and bio-aerosol samples from a wide variety of plants 4. Evaluation of the automated sandwich microarray immunoassay (LegioTyper) for rapid serotyping of Legionella pneumophila in agricultural exhaust air purification systems TUM is working on the following work packages: AP 1: Establishment of a Legionella rapid test for the investigation of exhaust air purification systems. AP 3: Use of the legionella rapid test to check the time and situation-dependent legionella growth in exhaust air purification systems over 1 year. AP 5: Use of the Legionella rapid test to check the time and situation-dependent growth of Legionella in exhaust air purification systems over 1 year.

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https://www.fisaonline.de/index.php?id=136&tx_fisaresearch_projects[p_id]=14318&tx_fisaresearch_projects[action]=projectDetails&tx_fisaresearch_projects[controller]=Projects&cHash=c80c9286a6da1d8958c1cba5deed093c Mon, 10 Aug 2020 08:07:16 +0000 https://www.fisaonline.de/index.php?id=136&tx_fisaresearch_projects[p_id]=14318&tx_fisaresearch_projects[action]=projectDetails&tx_fisaresearch_projects[controller]=Projects&cHash=c80c9286a6da1d8958c1cba5deed093c
Progress with competence and specialist knowledge for animal well-being, environmentally friendly and sustainable livestock farming In this project, suitability-tested and properly operated agricultural exhaust air treatment plants are to be examined for the presence of Legionella in order to be able to estimate the health risks to the population through the operation of these systems and to assess the need for action by lawmakers and law enforcement agencies to mitigate a potential risk. The following goals are pursued: • Recording the current status of exhaust air purification systems in livestock farming, which can potentially be contaminated with Legionella. • Risk assessment of exhaust air purification systems in livestock farming through regular sampling and examination for Legionella and amoeba in the process water and in the bio-aerosol-containing exhaust air (bio-aerosol) • Establishment of a quick and easy hygiene control using an automated microarray-based molecular biological rapid test (with integrated live / dead differentiation and upstream concentration method) for the culture-independent quantification of Legionella on site. • Evaluation of the measurement method with process water and bioaerosol samples from a wide variety of plants • Evaluation of the automated sandwich microarray immunoassay (LegioTyper) for rapid serotyping of Legionella pneumophila in agricultural exhaust air purification systems For this purpose, both a molecular biological isothermal rapid test for Legionella and an antibody-based rapid test for the identification of serogroups of Legionella pneumophila are established and analytically validated on the MCR R analysis platform. With this test the process water, biofilms and exhaust air from three representative properly operated ARAs are investigated for one year for Legionella. The planned method allows both the molecular biological quantification of bacteria of the genus Legionella and the health-relevant pathogen Legionella pneumophila, as well as molecular biological tests as to whether the bacteria found are viable or active or not, as well as antibody-based tests for the determination of serotypes of Legionella pneumophila that can differ in their pathogenicity. In a further step, this test will be used to investigate a representative cross-section of at least 25 ARA in Germany for Legionella. On the basis of this data, a risk assessment is created and a possible need for action is given.

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https://www.fisaonline.de/index.php?id=136&tx_fisaresearch_projects[p_id]=14317&tx_fisaresearch_projects[action]=projectDetails&tx_fisaresearch_projects[controller]=Projects&cHash=f63f82e213c5109ce372ef91b80fced9 Mon, 10 Aug 2020 08:04:26 +0000 https://www.fisaonline.de/index.php?id=136&tx_fisaresearch_projects[p_id]=14317&tx_fisaresearch_projects[action]=projectDetails&tx_fisaresearch_projects[controller]=Projects&cHash=f63f82e213c5109ce372ef91b80fced9
Funding of multifunctional and climate-stable forests through sustainable use of windthrow areas no details

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https://www.fisaonline.de/index.php?id=136&tx_fisaresearch_projects[p_id]=14316&tx_fisaresearch_projects[action]=projectDetails&tx_fisaresearch_projects[controller]=Projects&cHash=49f5da061b505cf19ec0a877bbd9ca72 Fri, 07 Aug 2020 08:53:44 +0000 https://www.fisaonline.de/index.php?id=136&tx_fisaresearch_projects[p_id]=14316&tx_fisaresearch_projects[action]=projectDetails&tx_fisaresearch_projects[controller]=Projects&cHash=49f5da061b505cf19ec0a877bbd9ca72
Investigation of animal welfare-environment-interaction of dairy cows. Subproject 2 In the context of the current discussion of surface areas (barn floors) within dairy freestall barns, as well as research topics on factors of animal welfare, this research task provides a common intersection. The surface areas polluted by excrements of the animals are mainly responsible for emissions of ammonia out of the barn. Furthermore, the surface area of the barn functions as a running surface for the animals and should provide a non-slip and safe footstep even under continuous load. The activity behavior and hoof health depends on the floor design. The characteristic feature 'lameness', the third most common reason of loss in German dairy farms, plays an important economic role of the floor design in addition to the factors animal welfare and health; therefore, with increasing importance of the durability of the animals and the dependent replacement costs. Currently, there are no additional studies on interactions of these target figures. The Research and Education Center Agriculture Haus Riswick of the Chamber of Agriculture North Rhine-Westphalia therefore is planning a new dairy barn to expand the possibilities of research and to focus on unique issues. The targets focus is on the interaction between animal welfare and environmental protection in dairy cattle husbandry. Simultaneously, the barn fulfills an important demonstration task at this research location, because many interested farmers visit Haus Riswick and discuss exemplary new and rebuild solutions with the experts. Therefore, the objective of this research project is to consider the three factors 'low emissions, high running comfort and long-living cows' in a three-year investigation phase. In the new experimental barn, important contributions and solutions for decision-making in science, consulting advice and practice are created. The proposed research project on animal welfare-environment-interaction of dairy cows is divided into five working packages. In a first step work packages AP 0 to AP3 are preconnected. During this period (01.01.2020 - 31.03.2021), planning, construction and commissioning of the new TUI research barn will take place by the Chamber of Agriculture of North Rhine-Westphalia. When planning and constructing the experimental barn, all structural requirements necessary for the issues specific for the research should be taken into account. At the beginning (AP 1) and at the end of the first research project (AP 5), expert discussions are planned in order to be able to discuss issues and research questions. The second step will be the application for the research project in 2020. In the three years of research (01.04.2021 - 31.03.2024) the measuring technique will be installed in the first year of the study (AP 4) and tested for its suitability during various daily and seasonal conditions. In the second experimental year (AP 5) experiments with different slatted floor variants are planned. On this occasion, different floor variants are simultaneously tested for their emission effects in different test compartments (case-control-study). At the same time, ethological considerations of animal behavior take place (AP 6). In the third year of investigation, targeted management changes will be made. The interactions of the floor with animal behavior will be evaluated and discussed and published as part of an expert workshop (AP 7 and AP 8).

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https://www.fisaonline.de/index.php?id=136&tx_fisaresearch_projects[p_id]=14315&tx_fisaresearch_projects[action]=projectDetails&tx_fisaresearch_projects[controller]=Projects&cHash=b296bb8a7254b4aa53ced60dc69f0db1 Fri, 07 Aug 2020 07:12:02 +0000 https://www.fisaonline.de/index.php?id=136&tx_fisaresearch_projects[p_id]=14315&tx_fisaresearch_projects[action]=projectDetails&tx_fisaresearch_projects[controller]=Projects&cHash=b296bb8a7254b4aa53ced60dc69f0db1
Investigation of animal welfare-environment-interaction of dairy cows. Subproject 3 In the context of the current discussion surface areas (barn floors) within dairy freestall barns, as well as research topics on factors of animal welfare, this research task provides a common intersection. The surface areas polluted by excrements of the animals are mainly responsible for emissions of ammonia out of the barn. Furthermore, the surface area of the barn functions as a running surface for the animals and should provide a non-slip and safe footstep even under continuous load. The activity behavior and hoof health depends on the floor design. The characteristic feature 'lameness”, the third most common reason of loss in German dairy farms, plays an important economic role of the floor design in addition to the factors animal welfare and health; therefore, with increasing importance of the durability of the animals and the dependent replacement costs. Currently, there are no additional studies on interactions of these target figures. The Research and Education Center Agriculture Haus Riswick of the Chamber of Agriculture North Rhine-Westphalia therefore is planning a new dairy barn to expand the possibilities of research and to focus on unique issues. The targets focus is on the interaction between animal welfare and environmental protection in dairy cattle husbandry. Simultaneously, the barn fulfills an important demonstration task at this research location, because many interested farmers visit Haus Riswick and discuss exemplary new and rebuild solutions with the experts. Therefore, the objective of this research project is to consider the three factors 'low emissions, high running comfort and long-living cows” in a three-year investigation phase. In the new experimental barn, important contributions and solutions for decision-making in science, consulting advice and practice are created. The proposed research project on animal welfare-environment-interaction of dairy cows is divided into two parts: In a first step work package 0 is preconnected. During this period (01.01.2020 – 31.12.2020), planning, construction and commissioning of the new TUI research barn will take place by the Chamber of Agriculture of North Rhine-Westphalia. When planning and constructing the experimental barn, all structural requirements necessary for the issues specific for the research should be taken into account. At the beginning and at the end of the research project, expert discussions are planned in order to be able to discuss issues and research questions. The second step will be the application for the research project in 2020. In the three years of research (01.01.2021 – 31.12.2023) the measuring technique will be installed in the first year of the study (AP 1) and tested for its suitability during various daily and seasonal conditions. In the second experimental year (AP 2) experiments with different slatted floor variants are planned. On this occasion, different floor variants are simultaneously tested for their emission effects in different test compartments (case-control-study). At the same time, ethological considerations of animal behavior take place (AP 3). In the third year of investigation, targeted management changes will be made. The interactions of the floor with animal behavior will be evaluated and discussed and published as part of an expert workshop (AP 4 and AP 5).

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https://www.fisaonline.de/index.php?id=136&tx_fisaresearch_projects[p_id]=14314&tx_fisaresearch_projects[action]=projectDetails&tx_fisaresearch_projects[controller]=Projects&cHash=efa46ff726a7414426faf0e9f3db9907 Fri, 07 Aug 2020 07:10:41 +0000 https://www.fisaonline.de/index.php?id=136&tx_fisaresearch_projects[p_id]=14314&tx_fisaresearch_projects[action]=projectDetails&tx_fisaresearch_projects[controller]=Projects&cHash=efa46ff726a7414426faf0e9f3db9907
Investigation of animal welfare-environment-interaction of dairy cows. Subproject 1 In the context of the current discussion surface areas (barn floors) within dairy freestall barns, as well as research topics on factors of animal welfare, this research task pro-vides a common intersection. The surface areas polluted by excrements of the animals are mainly responsible for emissions of ammonia out of the barn. Furthermore, the surface area of the barn functions as a running surface for the animals and should provide a non-slip and safe footstep even under continuous load. The activity behavior and hoof health depends on the floor design. The characteristic feature 'lameness', the third most common reason of loss in German dairy farms, plays an important economic role of the floor design in addition to the factors animal welfare and health; therefore, with increasing importance of the durability of the animals and the dependent replacement costs. Currently, there are no additional studies on interactions of these target figures. The Research and Education Center Agriculture Haus Riswick of the Chamber of Agriculture North Rhine-Westphalia therefore is planning a new dairy barn to expand the possibilities of research and to focus on unique issues. The targets focus is on the interaction be-tween animal welfare and environmental protection in dairy cattle husbandry. Simulta-neously, the barn fulfills an important demonstration task at this research location, be-cause many interested farmers visit Haus Riswick and discuss exemplary new and rebuild solutions with the experts. Therefore, the objective of this research project is to consider the three factors 'low emissions, high running comfort and long-living cows' in a three-year investigation phase. In the new experimental barn, important contributions and solutions for deci-sion-making in science, consulting advice and practice are created. The proposed research project on animal welfare-environment-interaction of dairy cows is divided into five working packages. In a first step work package 0 is precon-nected. During this period (01.01.2020 - 31.03.2021), planning, construction and commissioning of the new TUI research barn will take place by the Chamber of Agri-culture of North Rhine-Westphalia. When planning and constructing the experimental barn, all structural requirements necessary for the issues specific for the research should be taken into account. At the beginning (AP 0) and at the end of the first re-search project (AP 2), expert discussions are planned in order to be able to discuss is-sues and research questions. The second step will be the application for the research project in 2020. In the three years of research (01.04.2021 - 31.03.2024) the measuring technique will be installed in the first year of the study (AP 1) and tested for its suitability during various daily and seasonal conditions. In the second experimental year (AP 2) experiments with differ-ent slatted floor variants are planned. On this occasion, different floor variants are simultaneously tested for their emission effects in different test compartments (case-control-study). At the same time, ethological considerations of animal behavior take place (AP 3). In the third year of investigation, targeted management changes will be made. The interactions of the floor with animal behavior will be evaluated and dis-cussed and published as part of an expert workshop (AP 4 and AP 5).

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https://www.fisaonline.de/index.php?id=136&tx_fisaresearch_projects[p_id]=14313&tx_fisaresearch_projects[action]=projectDetails&tx_fisaresearch_projects[controller]=Projects&cHash=314ab65e2c5903dcb0298ed4547394f3 Fri, 07 Aug 2020 06:40:10 +0000 https://www.fisaonline.de/index.php?id=136&tx_fisaresearch_projects[p_id]=14313&tx_fisaresearch_projects[action]=projectDetails&tx_fisaresearch_projects[controller]=Projects&cHash=314ab65e2c5903dcb0298ed4547394f3
Collaborative project: National Animal Welfare Monitoring - subproject 8 Farm Animal Welfare is a controversial topic of growing public interest. At the same time, there is a lack of reliable information on the status quo of the welfare in German farm animals. Data on animal-based indicators for all relevant health areas (eg. lameness, injuries), animal behaviour and affectives states, resource-based indicators (eg. space allowance, feeing place, housing system) as well as management-based indicators (eg. feeding regime, duration of suckling period, outdoor access) are scarce and not collected systematically. Data that could be used to assess animal welfare are collected regularly (eg. meat inspection, milk recording scheme, antibiotic consumption), however, their use is limited due to: -    data not collected in a standardized manner -    data available only for specific types of production -    there is no legal basis for using data of eg. the animal trading databas (HIT) for the purpose of animal welfare assessment The aim of this project is to lay the foundations for a regular, indicator-based report on the welfare status of the most important small ruminant species in Germany, i.e. sheep and goats, and its evolution over time. This welfare monitoring will take into account several dimensions of animal welfare in the relevant life stages (husbandry, transport, slaughter). The following work packages will be included: 1.    Evaluation of existing information on a science-based preselection of potential welfare indicators, development of a synopsis 2.    Stakeholder analysis, selection of indicators with involvement of stakeholders 3.    Field-tests of suitability of indicators (survey on farms, transport, slaughterhouses), determination of data-collection costs if possible 4.    Data-processing for the animal welfare monitoring (incl. monitoring report) and assessment of the respective costs, if possible. 5.    Publication of a proto-type for an animal welfare monitoring report (print and online) 6.    Development of policy recommendations for the implementation of regular animal welfare monitoring The following events are planned to be conducted: -    project meetings at least twice a year -    information exchange with ongoing welfare projects and industry nationally as well as in the neighbouring countries: one event -    closing event together with the NaTiMon project

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https://www.fisaonline.de/index.php?id=136&tx_fisaresearch_projects[p_id]=14312&tx_fisaresearch_projects[action]=projectDetails&tx_fisaresearch_projects[controller]=Projects&cHash=893b90f37c8f00535599d9b5144a221c Fri, 07 Aug 2020 06:12:35 +0000 https://www.fisaonline.de/index.php?id=136&tx_fisaresearch_projects[p_id]=14312&tx_fisaresearch_projects[action]=projectDetails&tx_fisaresearch_projects[controller]=Projects&cHash=893b90f37c8f00535599d9b5144a221c
Legionella in agricultural exhaust air treatment plants. Subproject TUM TUM has the following goals in the project 1. Detection of the current status of exhaust air purification systems in livestock farming, which can potentially be contaminated with Legionella. 2. Establishment of a quick and easy hygiene control using an automated microarray-based molecular biological rapid test (with integrated live / dead differentiation and upstream concentration method) for the culture-independent quantification of Legionella on site. 3. Evaluation of the measurement method with process water and bio-aerosol samples from a wide variety of plants 4. Evaluation of the automated sandwich microarray immunoassay (LegioTyper) for rapid serotyping of Legionella pneumophila in agricultural exhaust air purification systems TUM is working on the following work packages: AP 1: Establishment of a Legionella rapid test for the investigation of exhaust air purification systems. AP 3: Use of the legionella rapid test to check the time and situation-dependent legionella growth in exhaust air purification systems over 1 year. AP 5: Use of the Legionella rapid test to check the time and situation-dependent growth of Legionella in exhaust air purification systems over 1 year

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https://www.fisaonline.de/index.php?id=136&tx_fisaresearch_projects[p_id]=14311&tx_fisaresearch_projects[action]=projectDetails&tx_fisaresearch_projects[controller]=Projects&cHash=2b8aefd36b5f23c6eba5f31b4d694ef1 Fri, 07 Aug 2020 05:58:37 +0000 https://www.fisaonline.de/index.php?id=136&tx_fisaresearch_projects[p_id]=14311&tx_fisaresearch_projects[action]=projectDetails&tx_fisaresearch_projects[controller]=Projects&cHash=2b8aefd36b5f23c6eba5f31b4d694ef1
Legionella in agricultural exhaust air treatment plants. Subproject TI In this project, suitability-tested and properly operated agricultural exhaust air treatment plants are to be examined for the presence of Legionella in order to be able to estimate the health risks to the population through the operation of these systems and to assess the need for action by lawmakers and law enforcement agencies to mitigate a potential risk. The following goals are pursued: • Recording the current status of exhaust air purification systems in livestock farming, which can potentially be contaminated with Legionella. • Risk assessment of exhaust air purification systems in livestock farming through regular sampling and examination for Legionella and amoeba in the process water and in the bio-aerosol-containing exhaust air (bio-aerosol) • Establishment of a quick and easy hygiene control using an automated microarray-based molecular biological rapid test (with integrated live / dead differentiation and upstream concentration method) for the culture-independent quantification of Legionella on site. • Evaluation of the measurement method with process water and bioaerosol samples from a wide variety of plants • Evaluation of the automated sandwich microarray immunoassay (LegioTyper) for rapid serotyping of Legionella pneumophila in agricultural exhaust air purification systems For this purpose, both a molecular biological isothermal rapid test for Legionella and an antibody-based rapid test for the identification of serogroups of Legionella pneumophila are established and analytically validated on the MCR R analysis platform. With this test the process water, biofilms and exhaust air from three representative properly operated ARAs are investigated for one year for Le-gionella. The planned method allows both the molecular biological quantification of bacteria of the genus Legionella and the health-relevant pathogen Legionella pneumophila, as well as molecular biological tests as to whether the bacteria found are viable or active or not, as well as antibody-based tests for the determination of serotypes of Legionella pneumophila that can differ in their pathogenicity. In a further step, this test will be used to investigate a representative cross-section of at least 25 ARA in Germany for Legionella. On the basis of this data, a risk assessment is created and a possible need for action is given.

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https://www.fisaonline.de/index.php?id=136&tx_fisaresearch_projects[p_id]=14310&tx_fisaresearch_projects[action]=projectDetails&tx_fisaresearch_projects[controller]=Projects&cHash=1086c0cb26cba0599a19f47f4ee133a4 Fri, 07 Aug 2020 05:53:09 +0000 https://www.fisaonline.de/index.php?id=136&tx_fisaresearch_projects[p_id]=14310&tx_fisaresearch_projects[action]=projectDetails&tx_fisaresearch_projects[controller]=Projects&cHash=1086c0cb26cba0599a19f47f4ee133a4
Comprehensive use of cocoa fruits currlently unavailable

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https://www.fisaonline.de/index.php?id=136&tx_fisaresearch_projects[p_id]=14309&tx_fisaresearch_projects[action]=projectDetails&tx_fisaresearch_projects[controller]=Projects&cHash=056ac15c9af9c209637d1910a6ce8fcf Thu, 06 Aug 2020 08:25:51 +0000 https://www.fisaonline.de/index.php?id=136&tx_fisaresearch_projects[p_id]=14309&tx_fisaresearch_projects[action]=projectDetails&tx_fisaresearch_projects[controller]=Projects&cHash=056ac15c9af9c209637d1910a6ce8fcf
Investigation and evaluation of the use of engineered nanomaterials in the food sector Nanotechnology applications are expected to bring changes to the food sector, including improved production and processing techniques, improved food contact materials, modification of taste and texture, monitoring food quality and freshness, reduced fat content, enhanced nutrient absorption, and improved traceability and security of food products. However, no clear information about the actual use of nanotechnology in the food industry is available and data on the benefits, improvements and risks of nanotechnology applications in the food sector as well as their economical competitiveness are still almost lacking. Therefore, a literature and patent search was started to fill the gaps and to identify future developments.

A variety of food ingredients, additives, encapsulation systems and food contact materials is already available in some countries and the market for nanotechnology-derived food products and food contact materials is expected to grow worldwide. Greiner R. (2008) Current and Projected Applications of Nanotechnology in the Food Sector. J. Braz. Soc. Food Nutr. 34, 243-260. Greiner R. (2009). Current and projected applications of nanotechnology in the food sector. Proceedings of the 8th Simpósio Latino Americano de Ciências de Alimentos, Campinas, Brazil, 21-22. Greiner R. (2009). Behaviour and fate of engineered nanomaterials following oral exposure. Proceedings of the 8th Simpósio Latino Americano de Ciências de Alimentos, Campinas, Brazil, 20. Greiner R. (2009) Verbleib und Verhalten synthetischer Nanomaterialien nach oraler Aufnahme. In: Nanotechnologie in der Lebensmittelindustrie (ed. Weber, H.), Behr’s Verlag, Hamburg, pp. 131-144. Greiner R. (2010) Advances in the Application of Nanotechnology in Food. Proceedings of the 22nd Brazilian Congress of Food Science and Technology (CD), Salvador, Brazil. Greiner R. (2010) Application of nanotechnologies in the food sector. Proceedings of the Max Rubner Conference 2010, Nanotechnology in the Food Sector. Max Rubner-Institut, Karlsruhe, Germany. Greiner R. (2010) Knowledge on the Behaviour of Engineered Nanomaterials Following Oral Exposure. Proceedings of the 15th IUFoST World Congress, Cape Town, South Africa, P0385. Greiner R. (2010). Activities in Food Nanotechnology at the Max Rubner-Institute to Protect the Consumer. NanoAgri 2010, São Pedro, Brazil. Greiner R. (2010) Do Engineered Nanomaterials in Food Pose a Health Risk? Proceedings of the IUFoST Conference “Using Science to Drive Success in the Food Market”, Warsaw, Poland. Greiner R. (2011) Current and projected applications of engineered nanomaterials in the food sector. Proceedings of the 3rd International Workshop on Advances in Science and Technology of Bioresources, Pucón, Chile, 157. Greiner R. (2011) Nanostructures Induced on Food Processing, Proceedings of the Fourth Nanotechnology Stakeholder Dialogue Day - ‘Potential food applications of nanotechnologies’, FoodDrinkEurope, Brussels, Belgium. Greiner R. (2011) Introducing Nanomaterials into the Food Sector: Repeating the Mistakes of GM Foods? Proceedings of the 3rd MoniQA International Conference, Varna, Bulgaria, 56. Greiner R. (2011) Nanotechnology in food and packaging materials. Proceedings of the 6th International Symposium on Turkey Production, 20-21. Greiner R. (2011) Nanotechnology in Food and Packaging Materials. In: Turkey Production and Health: An Update (ed. Hafez, H.M.), Mensch-Buch-Verlag, pp.91-103.Greiner R., Oehlke K. (2012) Anwendungen der Nanotechnologie im Lebensmittelbereich und Probleme der Lebensmittelsicherheit. RFL - Rundschau für Fleischhygiene und Lebensmittelüberwachung 5, 163-165. Greiner R., Oehlke K. (2012) Anwendungen der Nanotechnologie im Lebensmittelbereich und Probleme der Lebensmittelsicherheit. Leipziger Blaue Hefte 3, 488-490. Gräf V., Behsnilian D., Hetzer B., Walz E., Greiner R. (2013) Small Particles, Major Challenges – Nanomaterials in the Food Sector. GIT Laboratory Journal Europe 5-6/2013, 18-20. Oehlke K., Greiner R. (2013) Anwendungsmöglichkeiten für Nanomaterialien im Lebensmittelbereich. Ernährung im Fokus 3, 74-79. Oehlke K., Adamiuk M., Behsnilian D., Graef V., Mayer-Miebach E., Walz E., Greiner R. (2014) Potential bioavailability enhancement of bioactive compounds using food-grade engineered nanomaterials: a review of the existing evidence. Food & Function 5, 1341-1359. Gräf V., Walz E., Greiner R. (2014) Nanopartikel in Lebensmitteln unter die Lupe genommen. RFL - Rundschau für Fleischhygiene und Lebensmittelüberwachung 11, 398-400. Walz E., Gräf V., Greiner R. (2014) Nanotechnologie – eine Option für die Lebensmittelindustrie? Fleischwirtschaft 4, 58-60. Gräf V., Walz E., Greiner R. (2014) Charakterisierung von Nanomaterialien in Lebensmitteln: Herausforderungen und Lösungsansätze. Nanotechnologie aktuell 7, 106-110. Greiner R., Walz E., Gräf V. (2014) Kleine Teilchen, große Wirkung: Nanomaterialien im Lebensmittelsektor. Nanotechnologie aktuell 7, 102-105. Potential Economic Impact of Engineered Nanomaterials in Agriculture and the Food Sector in: Nanotechnology in Agriculture and Food Science. Axelos, Monique A. V. / Van de Voorde, Marcel (Herausgeber), Wiley, 1. Auflage März 2017 Oehlke, K., Behsnilian, D., Mayer-Miebach, E., Weidler, P. G., & Greiner, R. (2017). Edible solid lipid nanoparticles (SLN) as carrier system for antioxidants of different lipophilicity. PLOS ONE, 12, e0171662-. https://doi.org/10.1371/journal.pone.0171662

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https://www.fisaonline.de/index.php?id=136&tx_fisaresearch_projects[p_id]=14308&tx_fisaresearch_projects[action]=projectDetails&tx_fisaresearch_projects[controller]=Projects&cHash=b94ff2e93fe697e1dbe6e6038f1f0b0a Thu, 06 Aug 2020 06:37:41 +0000 https://www.fisaonline.de/index.php?id=136&tx_fisaresearch_projects[p_id]=14308&tx_fisaresearch_projects[action]=projectDetails&tx_fisaresearch_projects[controller]=Projects&cHash=b94ff2e93fe697e1dbe6e6038f1f0b0a
Giving Beekeeping Guidance by cOmputatiOnal-driven Decision making B-GOOD will pave the way towards healthy and sustainable beekeeping within the European Union by following a collaborative and interdisciplinary approach. Merging data from within and around beehives as well as wider socio-economic and ecological conditions, B-GOOD will develop and test innovative tools to perform risk assessments according to a novel Health Status Index (HSI). B-GOOD has the overall goal to provide guidance for beekeepers and help them make better and more informed decisions. During field-trials three sampling moments for the diagnosis of bee diseases will take place per year: a first in spring, when the bees start to forage; a second in summer, when the colonies have reached their maximum size; and a third in autumn before the over-wintering. The FLI will organize and perform diagnosis on these samples for Varroa-mites, Nosema spp., Deformed Wing Virus, spores of American foulbrood (Paenibacillus larvae) and the causative agent of European foulbrood (Melissococcus plutonius). If necessary additional lab tests, for instance when we suspect an infestation by Acarapis woodi (tracheal mite), or an infection by Acute or Chronic Bee Paralysis Virus, will be performed.

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Towards rational design of vaccines against African swine fever in East- and Southern Africa: Correlation of viral genome differences with their impact virulence and by analysis of viral and target cell transcription and protein expression. In many African countries the importance of pig breeding for nutrition and economy has increased substantially during the last decades. However, this development is inhibited by various animal diseases, among which African swine fever (ASF) is particularly problematic. While the infection is usually fatal in domestic pigs, the soft tick-borne African swine fever virus (ASFV) causes only mild symptoms in wart hogs and bush pigs, and is therefore widely distributed in these species, and transmitted to their domestic cognates. Although laboratory experiments suggest that vaccination against ASF should be possible in principle, development of a feasible vaccine was not successful up to now. Since ASFV isolates frequently exhibit different virulence, the complete genomes of several viruses of different genotypes, which are currently relevant in Africa, have to be compared. Representative isolates will be characterized in pigs, and the corresponding virus and host cell transcriptomes and proteomes will be analyzed using next generation sequencing and mass spectrometry. This might reveal a correlation between genetic markers (mutations leading to deletion or modified expression of viral and cellular genes) and virulence. To verify the biological relevance of the genome differences, they will be introduced by genetic engineering into closely related cell culture adapted ASFV strains. Therefore, reporter genes for fluorescent proteins (e.g. GFP) or other selectable markers will be transiently inserted into the ASFV genome, and state-of-the-art molecular biology techniques like genome modification by CRISPR/Cas (clustered regularly interspaced short palindromic repeats/Cas9 nuclease) will be applied. As soon as isogenic groups of virulent parental ASF viruses and recombinants with potential virulence gene mutations are available, their replication in vitro and their pathogenicity in vivo will be comparatively investigated. As far as possible, protective efficacy will be evaluated by subsequent challenge infections with virulent ASFV. The studies will include quantification of clinical symptoms and mortalities, pathological, virological and serological parameters (viral load in blood and organs, antibody production, leucocyte differentiation, cytokine and chemokine induction).

The project focused on a virulent ASFV isolate from Kenya (genotype IX), which could be easily adapted to efficient growth in a wild boar lung cell line. Next generation sequence analyses of the parental virus, and different cell culture passages revealed no significant genome alterations. Based on virulence studies and proteome analyses of other ASFV strains, it was attempted to delete known pathogenicity factors, and abundantly expressed virus genes from ASFV Kenya using improved reverse genetics methods. Whereas deletion of the gene encoding viral ribonucleotide reductase, membrane protein p12 and pB119L (9GL) did not result in replication competent virus mutants, the viral thymidine kinase- and dUTPase genes, as well as the abundantly expressed K145R und A104R genes could be removed successfully. Animal experiments will show, whether these mutations can contribute to development of attenuated live virus vaccines. In an alternative approach WSL cell lines stably expressing the CRISPR/Cas9 system with specific guide RNAs against ASFV Kenya. Since virus replication was severly inhibited in these cells, it will be evaluated whether transgenic, ASFV resistant swine can be generated in the same way.

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Collaborative project: Establishing digital indicators of bee vitality in agricultural landscapes. Subproject 9 In this project digital tools will be implemented in the field, in order to improve practical beekeeping as well as the evaluation of plant protection products by independent contract research organizations. These digital tools including an electronic bee counting device, automated beehive scales and an user-based software, are going to support the deduction of suitable digital bee keeping practices. Vitality of bees includes among others the ability to tolerate diseases and plant protection products in order to perform pollination and honey production. The new vitality indicators allow an automated measuring and assessment of bee vitality. Such vitality indicators shall be used by beekeepers to recognize vitality disturbances in their hives at an early stage, understanding the reasons for that and taking countermeasures. Contract research institutes could use the tools and vitality indicators to increase their efficiency and accuracy during their field trials.Regulatory authorities could include the vitality indicators to restate their safety goals as well as risk assessment and compensatory measures. Through a specific reference on the landscape, different cultivation methods can be assessed from the bee vitality perspective, as well as pollination performance. The digital tools including an electronic bee-flight activity counting device, automated bee scales and user-friendly software are going to support the dissemination of adequate bee-keeping practices. The application of the mentioned digital tools will be assured by a contract bee-research organization which develops and provides an innovative service for their clients. Another organization is going to assess the feasibilty of these services. Federal state institutes for beekeeping assuring the connection and knowledge-transfer to the beekeepers. The development of digital vitality indicators for honey bees will be implemented in a joint project with 10 partners as follows: beehives will be observed cont

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Collaborative project: Establishing digital indicators of bee vitality in agricultural landscapes. Subproject 8 In this project digital tools will be implemented in the field, in order to improve practical beekeeping as well as the evaluation of plant protection products by independent contract research organizations. These digital tools including an electronic bee counting device, automated beehive scales and an user-based software, are going to support the deduction of suitable digital bee keeping practices. Vitality of bees includes among others the ability to tolerate diseases and plant protection products in order to perform pollination and honey production. The new vitality indicators allow an automated measuring and assessment of bee vitality. Such vitality indicators shall be used by beekeepers to recognize vitality disturbances in their hives at an early stage, understanding the reasons for that and taking countermeasures. Contract research institutes could use the tools and vitality indicators to increase their efficiency and accuracy during their field trials. Regulatory authorities could include the vitality indicators to restate their safety goals as well as risk assessment and compensatory measures. Through a specific reference on the landscape, different cultivation methods can be assessed from the bee vitality perspective, as well as pollination performance. The digital tools including an electronic bee-flight activity counting device, automated bee scales and user-friendly software are going to support the dissemination of adequate bee-keeping practices. The application of the mentioned digital tools will be assured by a contract bee-research organization which develops and provides an innovative service for their clients. Another organization is going to assess the feasibilty of these services. Federal state institutes for beekeeping assuring the connection and knowledge-transfer to the beekeepers. The development of digital vitality indicators for honey bees will be implemented in a joint project with 10 partners as follows: beehives will be observed cont

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Collaborative project: Establishing digital indicators of bee vitality in agricultural landscapes. Subproject 7 In this project digital tools will be implemented in the field, in order to improve practical beekeeping as well as the evaluation of plant protection products by independent contract research organizations. These digital tools including an electronic bee counting device, automated beehive scales and an user-based software, are going to support the deduction of suitable digital bee keeping practices. Vitality of bees includes among others the ability to tolerate diseases and plant protection products in order to perform pollination and honey production. The new vitality indicators allow an automated measuring and assessment of bee vitality. Such vitality indicators shall be used by beekeepers to recognize vitality disturbances in their hives at an early stage, understanding the reasons for that and taking countermeasures. Contract research institutes could use the tools and vitality indicators to increase their efficiency and accuracy during their field trials. Regulatory authorities could include the vitality indicators to restate their safety goals as well as risk assessment and compensatory measures. Through a specific reference on the landscape, different cultivation methods can be assessed from the bee vitality perspective, as well as pollination performance. The digital tools including an electronic bee-flight activity counting device, automated bee scales and user-friendly software are going to support the dissemination of adequate bee-keeping practices. The application of the mentioned digital tools will be assured by a contract bee-research organization which develops and provides an innovative service for their clients. Another organization is going to assess the feasibilty of these services. Federal state institutes for beekeeping assuring the connection and knowledge-transfer to the beekeepers. The development of digital vitality indicators for honey bees will be implemented in a joint project with 10 partners as follows: beehives will be observed cont

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https://www.fisaonline.de/index.php?id=136&tx_fisaresearch_projects[p_id]=14303&tx_fisaresearch_projects[action]=projectDetails&tx_fisaresearch_projects[controller]=Projects&cHash=316f0f821fc8a4a105af1afcae3d2216 Wed, 05 Aug 2020 12:36:51 +0000 https://www.fisaonline.de/index.php?id=136&tx_fisaresearch_projects[p_id]=14303&tx_fisaresearch_projects[action]=projectDetails&tx_fisaresearch_projects[controller]=Projects&cHash=316f0f821fc8a4a105af1afcae3d2216
Collaborative project: Establishing digital indicators of bee vitality in agricultural landscapes. Subproject 6 In this project digital tools will be implemented in the field, in order to improve practical beekeeping as well as the evaluation of plant protection products by independent contract research organizations. These digital tools including an electronic bee counting device, automated beehive scales and an user-based software, are going to support the deduction of suitable digital bee keeping practices. Vitality of bees includes among others the ability to tolerate diseases and plant protection products in order to perform pollination and honey production. The new vitality indicators allow an automated measuring and assessment of bee vitality. Such vitality indicators shall be used by beekeepers to recognize vitality disturbances in their hives at an early stage, understanding the reasons for that and taking countermeasures. Contract research institutes could use the tools and vitality indicators to increase their efficiency and accuracy during their field trials. Regulatory authorities could include the vitality indicators to restate their safety goals as well as risk assessment and compensatory measures. Through a specific reference on the landscape, different cultivation methods can be assessed from the bee vitality perspective, as well as pollination performance. The digital tools including an electronic bee-flight activity counting device, automated bee scales and user-friendly software are going to support the dissemination of adequate bee-keeping practices. The application of the mentioned digital tools will be assured by a contract bee-research organization which develops and provides an innovative service for their clients. Another organization is going to assess the feasibilty of these services. Federal state institutes for beekeeping assuring the connection and knowledge-transfer to the beekeepers. The development of digital vitality indicators for honey bees will be implemented in a joint project with 10 partners as follows: beehives will be observed cont

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