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Further Risk Management fort the EC listed Anoplophora Species, A. chinensis and A. glabripennis (ANOPLORISK II)
Project
Project code: 2813ERA001, JKI-AG-08-1149
Contract period: 01.10.2014
- 30.10.2015
Budget: 49,976 Euro
Purpose of research: Applied research
Keywords: Asian long-horned beetle, Citrus long-horned beetle
The project is part of an EUPHRESCO project with partners from the UK and Austria (ANOPLORISK II). Aim of the global project is the further development of risk managment of the Anoplophora species, A. chinensis and A. glabripennis which are listed as quarantine organisms in the EU. Sub-goals for the JKI-part are 1. practical application of an isothermal amplification based molecular identification method (LAMP) using a portable machine (GENIE II) for Anoplophora glabripennis (ALB) and A. chinensis (CLB) and 2. the development of contingency plans for the mentioned Anoplophora species. Plan: Concerning goal 1: At start of the project a technology transfer will be carried out with the UK partner FERA. FERA took place in an EU-research project during which primers to be used with GENIE II were developed. During this scientific exchange information on the primers will be given and arrangements for their improvement (primer design) will be carried out. In the following, the method will be be tested using the developed primers under practical applications. Concerning 2. based on the recommendations for eradicating ALB and CLB by the EU-Commission, the European Plant Protection Organization EPPO, contingency plans will be developed taking into account those used in disaster control or fighting against animal diseases. The following parts will be covered: a ) biology, b) experiences in eradication campaigns, c) legal background, d) diagnosis, e) eradication, f) regional general committments, g) public awareness campaigns and e) finances. Results: concerning 1: Development of guidelines for the national plant protection services for the use of teh system at border controlls. Concerning 2: Preparation of contingency examples for the plant protection services of the EU-memberstates. The current project aims the establishment and validation of the innovative molecular biological method of a loop mediated isothermal amplification (LAMP) . The technique should be applied for the detection of different species of the genus Anoplophora- A. chinensis (CLB) and A. g/abripennis (ALB) as weil as for native Ianghorn beetle species. The method should support plant protection services and their diagnostic features in terms of a rapid detection of these quarantine organisms at import control as weil as during monitaring activities. ln tight collaboration between the project partners from FERA and the JKI, a set of primers for LAMP ALB and CLB detection was developed . The primer's specificity was in silico tested against DNA sequences from phylogenetically and morphologically related species. These tests based on camparisans within an own sequence data base derived and compiled from different publicly available sequence data bases. Furthermore a number of fresh samples from ALB and CLB larvae was provided from colleagues at the Bavarian State Research Centre for Agriculture and the ltalian Fondazione Minoprio.
Section overview
Subjects
- Crop Protection
- Forestry
Framework programme
Funding programme
Excutive institution
Institute for National and International Plant Health (JKI-AG)
