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Fire blight resistance of the wild apple species Malus fusca - mapping, gene isolation and functional characterization
Project
Project code: JKI-ZGO-08-4183
Contract period: 01.10.2015
- 30.09.2018
Purpose of research: Applied research
Keywords: E. amylovora, resistance mapping, minor QTLs, phenotyping
The objective of this research proposal is to isolate and functionally characterize the gene(s) underlying Mfu10. A population of 1,336 progeny individuals was established for a fine mapping approach. Genotyping with closely linked SSR markers has allowed for the identification of individuals showing recombination within the QTL interval. Recombinant individuals will form the basis for the isolation of the gene. To study the interaction of Mfu10 with the avrRpt2EA effector, the mapping population will be phenotyped with ZYRKD3-1 and an avrRpt2EA deletion mutant of strain Ea3049 and the respective complemented strain. Additionally, preliminary results suggest that another resistance locus in M. fusca or minor QTLs are contributing to the resistance of M. fusca. Therefore, to precisely detect these loci, the completion and saturation of the M. fusca genetic map is necessary. Overall, it is expected that the underlying resistance genes of M. fusca will provide resistance against the highly virulent E. amylovora isolates responsible for the breakdown of the Mr5 QTL and that pyramiding of these factors together with FB_MR5 will provide more durable resistance.
Section overview
Subjects
- Plant Breeding
- Crop Protection
- Arboriculture
Framework programme
Funding programme
Excutive institution
Institute for Breeding Research on Horticultural Crops (JKI-ZG)