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Programmable REGulation of gene activity in HAPLOid microspores for improved crop plant breeding (HaploReg)
Project
Project code: 031B0555
Contract period: 01.07.2018
- 30.06.2020
Budget: 499,960 Euro
Purpose of research: Basic research
Precision breeding to integrate specific, agriculture-relevant traits for applications is highly demanded to secure agricultural production in world’s changing climate and growing population. Currently, precise genome editing in plants has been achieved only in somatic cell models of various origin. Development of a technology to directly edit genome of haploid microspores (immature male generative plant cells) in a controllable manner will enable rational mutagenesis and generation of fully homozygous plants for breeding programs in a single step by doubling genome of genome-edited microspores. A major bottleneck for application of novel biotechnologies even in crops with the established microspore embryogenesis protocols is a lack of efficient methods for delivery of macromolecules. Therefore, this project targets development of efficient uptake of macromolecules in microspores from rapeseed and maize for subsequent validation and use of various components of genome editing (GE) machinery in multiplex/high-throughput assays. The proof-of-concept genome-editing applications in microspores will be extended to targeting non-coding regions of the genome, in particular miRNA genes, for programmable simultaneous repression and expression of protein sequences
Section overview
Subjects
- Biotechnology
- Process engineering
