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Collaborative project: Development of a lateral flow assay (LFA) for the simultaneous detection of four bee pathogenic viruses - subproject A (LAFAS)
Project
Project code: 281C302A19
Contract period: 01.02.2021
- 31.03.2024
Budget: 377,756 Euro
Purpose of research: Experimental development
Keywords: animal health, virology, monitoring, diagnostics, bees
The aim of the project is the development of a lateral flow assay (LFA) for the simultaneous detection of the four bee pathogenic viruses DWV, ABPV, CBPV and BQCV. The assay will offer the possibility of qualitative (personal, home use) as well as quantitative (specialist, professional) virus determination and will therefore be widely applicable, both for self-monitoring by the beekeeper and for virus screening by diagnostic laboratories in the context of e.g. monitoring programs. An early diagnosis of viral infections in individual colonies, which in the best case scenario takes place before the appearance of symptoms or signs of colony losses, gives the beekeeper the opportunity to use beekeeping measures to strengthen the immune status of the affected colonies or to improve the health of the colonies not yet affected by using targeted quarantine measures to prevent the spread of the infection in the apiary. Against this background, a fast, specific and cost-effective detection method for relevant bee viruses is urgently needed. A promising approach for such a detection of viral pathogens is the development of the immunological test system planned in this project, as it is widely used for the detection of viral pathogens in human and veterinary medicine. Immunological test systems are characterized by high specificity and sensitivity and also ensure fast and inexpensive detection of the viral pathogens even with high sample throughput, as can occur in the context of monitoring programs or when screening large (professional) beekeeping operations. Inexpensive methods that allow a high throughput are of great importance, especially for carrying out preventive examinations of clinically inconspicuous bee colonies.
Section overview
Subjects
- Beekeeping and health
