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Collaborative project: FUGATO-plus - FEPROeXPRESS - Fetal Programming by dietary protein deficit and surplus: relevance to the characteristics associated with productivity, tissue-specific gene and protein expression in pigs; Subproject Bonn University
Project
Project code: 0315132B
Contract period: 01.02.2008
- 31.01.2011
Budget: 140,218 Euro
Purpose of research: Basic research
'Intrauterine growth is a long known problem in the livestock industry and is compatible with economic losses and problems of acceptance on the part of consumers. Studies in rodents have shown that the offspring of inadequate during pregnancy mothers fed reduced birth weight and postnatal long-lasting effects in terms on the immune status such as growth, body composition and metabolic parameters associated deteriorated as glucose tolerance, insulin resistance, elevated glucose production and changes in energy metabolism. Also at the pig farm, piglets with a lower birth weight in comparison to medium piglets decreased muscle growth, increased fat and inferior meat quality.
Against this background, in a model study on the hog at the graviden gilts isoenergetic effects of three diets with varying protein content (6%, 12%, 30%) on productivity characteristics of the offspring will be investigated. Both low-and high-protein diet leads to intrauterine growth retardation. Besides the birth weight, growth parameters, body composition and plasma metabolites in a 28 (spreader) and 185-day-old offspring investigated and this in relation to gene-(microarray analysis) and protein expression (2D electrophoresis, and peptide mass fingerprinting by MALDI TOF-MS) in liver, muscle and fat. In addition, also of fetal tissue from the 95th gestation day won. This serves to diet-related, tissue-specific signaling and function of molecules to identify the level of productivity features observed changes are based. In addition, the diet-related displacement of the selected candidate molecules somatotropen axis (eg IGF-1, IGF-binding protein, insulin, Leptin) in plasma and tissue levels (ELISA, Western blot ligand) will be investigated. In the case of enzymes, in vitro enzyme activity assays established. Finally, to clarify whether long-lasting effects on the transcriptome and performance or metabolic parameters on epigenetic modifications of genes (DNA methylation) are due.
We expect with the results from this project, fundamental insights to diet-related, fetal initiation of postnatal development, growth and health as well as on possible mechanisms of growth to win. In addition, this project will contribute to the elucidation of molecular pathways of genotype-environment (diet)-interaction in pigs and provides insights into the mechanisms of adaptation and growth, which ultimately lead to a variation of birth weight. It will also identify candidate genes, can influence the prenatal growth and the ability of adaptation to intrauterine nutrient environment.'
Section overview
Subjects
- Animal breeding
- Animal nutrition
Collaborative Project
FUGATO-plus Collaborative Projects– Functional Genome Analysis in Animal Organisms
Framework programme
BMBF - Frameworkprogramm Biotechnology - Using and Shaping its Opportunities
