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LIGNIN METABOLIC PATHWAY - Impact of modifications in lignin metabolic pathways on fungal and bacterial pathogen resistance using Arabidopsis mutants and transgenic lines
Project
Project code: 0313096
Contract period: 01.08.2004
- 30.10.2007
Budget: 125,780 Euro
Purpose of research: Basic research
The objectives of the project are to use Arabidopsis lines deregulated (under- or over-expressing) in the lignin biosynthetic pathway to determine the role of cell wall bound and soluble phenolic compounds in pathogen resistance. A collection of lines in the same ecotype has been obtained by Partner 1. The detailed consequences on lignin content and composition have been determined in the frame of previous programs (Génoplante and Agrice). Preliminary studies have shown that, in addition to direct consequences on lignin characteristics, these deregulations can induce modifications in the content and composition of soluble phenolics. These lines will be used to determine the consequences of the modifications of the cell-bound and soluble phenolic pools on bacterial, oomycete and fungal pathogens. The chosen pathogens are bacterial (Pseudomonas syringae and Xanthomonas campestris), oomycete (Peronospora parasitica and Phytophthora parasitica) biotrophic pathogens as well as fungal necrotrophic pathogens (Alternaria brassicicola and Botrytis cinerea as well as Plectosphaerella cucumerina). These bioassays will be performed in different laboratories (Partners 2 to 5) according to the respective competences on Arabidopsis/ pathogen interactions. These studies will allow to determine the exact impact of each gene deregulation. These results will be compared to those obtained on phenolic content and on digestibility parameters (performed in the frame of Génoplante 2) and will allow a larger knowledge of deregulation consequences of this metabolic pathway. The results of this project will give major information in the potential interest of lignin pathway deregulation in crops. The main objective being to answer the question: is it possible to improve digestibility parameters without negative impact on pathogen resistance.
Section overview
Subjects
- Plant Breeding
Framework programme
BMBF - Frameworkprogramm Biotechnology - Using and Shaping its Opportunities
