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In situ detection and characterization of organic nanoscale and microscale structures in biological matrices using scanning electron spectroscopy
Project
Project code: MRI-LBV-08-1032 Rasterelektronenmikroskop organisc
Contract period: 01.01.2016
- 31.12.2020
Purpose of research: Applied research
For classical scanning electron microscopic examination of hydrous biological samples (e.g., food, microorganisms, plants), these samples must be subjected to chemical fixation and dehydration for classical SEM analysis in order to be stable in the high vacuum of the SEM sample chamber. Associated artifacts (unwanted object shrinkage, destruction of sensitive biological fine structures) often complicate the subsequent SEM analysis or do not reflect the natural state of the sample. In the meantime, modern SEM detectors or preparation methods have been developed that enable the visualization of aqueous biological samples with optimal image quality and object fidelity. Another focus of the project is the application of these modern in situ SEM methods for biological samples in the food sector. One is the ESEM mode and the cryo-REM mode. In order to determine the appropriate sample preparation method, comparative studies with the various available analysis variants are carried out and compared with the results of classical sample preparation with biological samples (chemical fixation and drying).
Braun, H., Woitsch, L., Hetzer, B., Geisen, R., Zange, B. & Schmidt-Heydt, M. (2018). Trichoderma harzianum: Inhibition of mycotoxin producing fungi and toxin biosynthesis at a transcriptional level. International Journal of Food Microbiology. Danylec, N., Göbl, A., Stoll, D. A., Hetzer, B., Kulling, S. E. & Huch, M. (2018). Rubneribacter badeniensis gen. nov., sp. nov. and Enteroscipio rubneri gen. nov., sp. nov., new members of the Eggerthellaceae isolated from human faeces. International Journal of Systematic and Evolutionary Microbiology. Wiedenmann, V., Oehlke, K., van der Schaaf, U., Hetzer, B., Greiner, R. & Karbstein, H. P. (2018). Impact of the incorporation of solid lipid nanoparticles on β-lactoglobulin gel matrices. Food Hydrocolloids.
Section overview
Subjects
- Biotechnology
- Food microbiology
Framework programme
Funding programme
Excutive institution
MRI - Department of Food and Bio Process Engineering (MRI-LBV)
