We use cookies on our website. Some are necessary for the operation of the website. You can also allow cookies for statistical purposes. You can adjust the data protection settings or agree to all cookies directly.
Data Protection Declaration
Imprint
Characterization and optimization of the replication of hepatitis E virus in cell culture, a project of the German One Health Initiative (GOHI)
Project
Project code: BfR-BIOS-08-1322-686
Contract period: 01.01.2018
- 31.12.2019
Purpose of research: Applied research
The notified hepatitis E cases significantly increased in Germany during the past years. The hepatitis E virus (HEV) infection is a zoonosis, which can be transmitted through contact to infected pigs and wild boars or through consumption of food prepared thereof. Several basic questions of virus replication as well as aspects of virus stability or antiviral substances could not be invetigated so far due to the lack of efficient cell culture systems for HEV. However, the recent isolation of better replicating strains should now allow such studies in principle. In the proposed project, the replication of HEV strain 47832c, which was originally isolated and adapted to cell culture at the BfR, will be investigated in more detail and optimized. To this end, basic aspects like the morphology of virus particles and the kinetics of replication and gene expression will be investigated in a first step. A reverse genetics system, which should allow the insertion of mutations and markers, will also be developed for the strain 47832c. Using this system, the significance of a distinct genome insertion will be investigated and the virus replication and its analysis will be enhanced. The optimized cell culture system will be available for analysis of specific questions raised by the project partners after the end of the project. The investigations should give insights into basic processes of HEV replication, but also provide robust and efficient cell culture systems for use in applied science. This project is cooperation project embedded into the German One Health Initiative (GOHI).
Section overview
Subjects
- Animal health
- Biotechnology
- Toxicology
