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Collaborative project: Extension of the oilseed rape gene pool by introgression of effective pest resistance from related species for an effective oilseed rape breeding in future - subproject B (WildResRaps)
Project
Project code: 281D121B21
Contract period: 01.08.2023
- 31.07.2026
Budget: 358,804 Euro
Purpose of research: Applied research
Keywords: plant breeding, plant genetic resources, crop protection, plant health, resistance, animal pathogens, crop production, rape seed
The aim of this project proposal is to significantly reduce the barriers of crossability with related species and the generation of recombinant chromosomes in order to be able to use wild, related but fundamentally crossable species more effectively e.g., to improve insect resistance in the oilseed rape gene pool. The scientific and technical work goals of our project can be divided into the following three parts: 1) Production of interspecific hybrids from B. napus/B. rapa and foreign species with resistance to cabbage stem flea beetle. The aim is to identify B. rapa genotypes that exhibit lower barriers to interspecific hybridization. In addition to the manual/insect-related crossings, the protoplast fusion is optimized in a further work package in order to effectively generate somatic hybrids in tissue culture. 2) Increasing the recombination rate between the recipient genome and the donor chromosomes to generate as many and as different as possible introgression lines. Undirected DNA double-strand breaks in B. napus/B. rapa hybrids with a foreign genome are induced and the frequency and distribution of the recombination events are measured. Applied to the material generated under 1), genetically stable introgression lines will be generated, which can be tested for cabbage stem flea beetle resistance using a biotest. 3) Establishment of a nuclease-induced chromosome recombination as a proof-of-concept. The insertion of targeted double-strand breaks in the recipient and donor genome using the sgRNA/nuclease complex in hybrid cells is intended to promote recombination of the donor fragments.
Section overview
Subjects
- Plant Breeding
- Crop Protection
- Genetic Resources
Funding programme
Excutive institution
Funding institution
Project management agencies
Associated projects: WildResRaps
- Collaborative project: Extension of the oilseed rape gene pool by introgression of effective pest resistance from related species for an effective oilseed rape breeding in future - subproject A
- Collaborative project: Extension of the oilseed rape gene pool by introgression of effective pest resistance from related species for an effective oilseed rape breeding in future - subproject C
