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Collaborative project: Development of a PCR based rapid test for the quantitative allergen monitoring in the entire food production chain – Subproject 3

Project


Project code: 2816401108
Contract period: 01.09.2009 - 31.08.2012
Budget: 218,434 Euro
Purpose of research: Experimental development

QUALLITÄT is a R&D project which focuses on the development and validation of a PCR based modular rapid tests for the screening and the subsequent quantitative analysis of al-lergens covered by the EU declaration regulation. The CONGEN Biotechnologie GmbH is responsible for Sample preparation/DNA-Isolation and purification, reference-DNA and the quantitative real-time PCR. The Bavarian Health and Food Safety Authority is developing a molecular based screening system for the detection of allergens that are subject to declaration. For this purpose, spe-cies-specific DNA-fragments suitable for identification need to be located in the genome. Compared to current methods, the new method holds out the distinct advantage that all allergens that are subject to declaration can be detected in only one analysis. In a second step, a quantitative determination of each allergen is possible. The production of reference materials is the task of the State Institute for Chemical and Veterinary Analysis of Food, Freiburg. Typical food matrices as sausages or bakery products shall be manufactured and spiked with all food allergens in the relevant, very low concentration level. Finally, the use of these materials shall allow a quantification of food allergens.

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