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Transkriptomprofilierung of embryo and endometrial biopsy based on the Trächtigkeitserfolg after transfer of in vitro and in vivo produced bovine embryos
Project
Project code: 53162125
Contract period: 01.01.2007
- 31.12.2010
Purpose of research: Basic research
Embryonic mortality accounts for the largest proportion of reproductive wastage in cattlebreeding. The main goal of this project is to determine the transcriptional profile of bovineembryos and endoemtrium at early stage of development in relation to pregnancy successafter transfer of in vitro- or vivo-derived blastocysts. For this biopsied embryos will transferredto synchronized recipients on day 7 after estrus and until day 24 blood plasma concentrationswill be measured and pregnancy status of the recipients will be determined and animals will becategorized to pregnant (with rapid increase in plasma level) and nonpregnant (slow increasein plasma level). Six animals from each group will be slaughtered at day 16 and embryo andendometrium samples will be collected for analysis. Animals with rapid increase in plasmaprogestron concentration are further followed up and ultrasound guided pregnancy control willbe done on day 28. Six animals from pregnant and six animals from nonpregnant animals willbe subjected to slaughter and embryo samples will be collected for further analysis.Transcriptional profile of blastocyst biopsies collected perior to transfer will be performedbased on the pregnancy success after transfer. Moreover, comparative transcriptional analysisof in vitro versus in vivo derived embryos at days 16 and 28 of gestation will be determinedusing bovine Affymetrix array (~22,000 ESTs). The endometrial biopsies obtained prior totransfer will also be used for transcriptional and proteomic analysis based on the pregnancysuccess after transfer. With this it is possible to draw the expression profile of embryos at thetime of transfer (using blastocyst biopsies collected) and during the course of pregnancy(day16 and 28). Comparative anaylsis of these expression profiles will enable to identifycandidate genes and draw pathways related to embryo survival after transfer to recipients.Moreover, the functional role of some of the candidate genes in early embryonic developmentwill be investigated using RNA interefence and polymorphism of the candidate genes at singlenucleotide level will be determined to associate with the fertility trait of the bull i.e. the NonReturn Rate (NRR).
Section overview
Subjects
- Animal breeding
