A combination of systems biology and experimental high-throughput approaches to engineer durable resistance against plant viruses in crops (COBRA)
Project code: JKI-RS-08-3374
Contract period: 01.07.2014 - 30.11.2017
Purpose of research: Applied research
To reach these goals, all information available for host virus interaction in barley, peach and tomato will be stored in a data bank and used to identify candidate genes. Besides this, new candidate genes will be identified by positional cloning in Arabidopsis and barley. Candidate genes will be tested and verified by complementation analyses, mutation analyses and screening for natural diversity. For selected candidate genes studies on host virus interaction will be conducted.
Plant viruses cause high yield losses in agriculture every year. Direct control of virus diseases is not possible, thus breeding for resistant varieties is the only way to prevent yield and quality losses. COBRA points on a combined use of systems biology approaches and high throughput technologies to identify host factors needed for an efficient virus infection (candidate genes for susceptibility) in barley, tomato and peach. In order to isolate rym13, amarker data were aligned to the reference sequence of barley and promising candidate genes were mapped in two mapping populations. Regarding Ryd3 the mapping population was extended to around 7400 F2 plants. By phenotyping newly developed RILs no further reduction of the interval of resistance was achieved. The resolution will be extended by phenotyping additional RILs in 2017. Furthermore different accessions from the genebank in Gatersleben and descendants of a TILLING population were screened for resistance. 40 out of 178 genebank accessions turned out to be resistant either to both viruses or to one of the viruses of the barley yellow mosaic virus complex (BaMMV/BaYMV). Regarding the TILLING population one genotype was confirmed to be resistant by mechanical inoculation with BaMMV in the growth chamber. Furthermore, 461 plants showed no infection in the field with BaMMV or BaYMV in the field and will be tested by mechanical inoculation in the growth chamber