Collaborative project: Establishment and validation of analytical methods for the detailed analysis of allergen profiles in foodstuffs and for the individual characterisation of its allergenic effect in patients - subproject C (ALLERGEN-PRO)
Project code: 281A304C18
Contract period: 01.09.2020 - 31.08.2023
Budget: 198,042 Euro
Purpose of research: Applied research
Keywords: food analysis, allergens, allergy/incompatibility response, consumer information, product safety, food safety, diagnostics, cause and effect-relationship
The project ALLERGEN-PRO has the overall goal to develop improved analytical methodologies based on PCR and mass spectrometric tools for the unambiguous identification and quantification of allergenic compounds from insects that are relevant for food production. The developed methods will be used by project partners in their routine analysis and proposed for enforcement purposes by official control laboratories. In addition innovative in vitro tests for the identification of allergen IgE/IgG epitopes in the insect proteome are developed. Those methods will improve food safety by providing the possibility for patients with allergic cross-reactions to shellfish and house dust mites to test a potential allergy to insects. For the first time a whole blood diagnostic system will be developed that will allow patients without any risk to determine if an allergy has been manifested or if it is just an intolerance without clinical symptoms. The new in vitro diagnostic tool will be used for the detection of clinically relevant allergic reactions in patients allergic against house dust mite using antigen–specific reaction of immune cells. The establishment of such diagnostic tools for different processed allergens in foods will be of benefit for patients to be informed about a clinically relevant food allergy. This research project will also deliver information about the clinical relevance of insect as a potential allergic food product. The project is divided in 6 work packages. In the project ALLERGEN-PRO qualitative and quantitative methods for the detection of insects and potential allergens thereof, respectively, developed and validated. In addition new in vitro diagnostic tools for the detection of IgE binding profiles and in vitro test systems established which will identify a clinically relevant food allergy.