Species identification in meat, meat products and meat meals
Project code: MRI-FL-08-4001
Contract period: 01.01.2001 - 31.12.2020
Purpose of research: Applied research
In the past species identification was performed by means of electrophoretical and immunological methods. Nowadays polymerase chain reaction (PCR) is more and more used and it is possible to differentiate clearly within the main animal species cattle, pig, sheep and poultry in raw up to highly heated products. There still exists considerable need of research with respect to the differentiation within the group of poultry, more rare domestic animals like goat, horse, donkey, pets, wild living and exotic animal species. Using suitable mitochondrial and nuclear DNA sequences it is planned to find ways to differentiate within the mentioned groups. Meat meal due its high heating causes still problems in detection.
A primer system was found so far which allows in the promotor region of the single copy gene beta casein for pig and cattle as well as by means of the single copy gen neuroglobine for goat the clear differentiation between goat, pork, beef and other relevant animal species used for meat products. Meanwhile it is possible to differentiate the most common poultry species like gosse, duck, turkey, guinea fowl, quail, chicken and pheasant by mitochondrial cytochrome b gene. Furthermore cattle, bison and buffalo can be also distinguished using specific primer systems