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Abundance and diversity of clostridia in agricultural biogas plants with particular attention to Clostridium botulinum
Project
Project code: ML 105.1, TI-BD-08-PID1576
Contract period: 01.09.2011
- 31.12.2014
Purpose of research: Applied research
Clostridia are bacteria which are highly abundant in biogas production plants during the fermentation of organic substances. Is it possible, that among those bacteria, also problematic clostridia could grow? There are problematic bacteria within the group of Clostridia, these include Clostridium botulinum, the producer of the most toxic biological susbstance known. The objective of this study was to assess the diversity of clostridia in substrates and fermentating material connected to experimental and agricultural biogas production processes. - Extraction of nucleic acids from substrates and fermentation material collected from experimental and agricultural biogas production processes - PCR Amplification of Clostridium cluster I sequences - Sequencing of PCR products by means of next-generatíon DNA sequencing tools (454 pyrosequencing, Illumina MySeq) - Bioinformatic and phylogenetic analyses of DNA sequences - Screening of environmental samples and identification of those with potential risks due to the presence of rRNA genes of suspected pathogens - Confirmation of presence of C. botulinum by mouse lethality tests (out of house).
Section overview
Subjects
- Process engineering
- Renewable Resources